Quantitative analysis of spinothalamic tract neurons in adult and developing mouse.
Quantitative analysis of spinothalamic tract neurons in adult and developing mouse.
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DOI：
10.1002/cne.22392
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发表时间：
2010-08-15
期刊：
影响因子：
--
通讯作者：
Giesler GJ Jr
中科院分区：
文献类型：
--
作者：
Davidson S;Truong H;Giesler GJ Jr
了解伤害感觉回路的发展对于正确治疗疼痛和对产前、新生儿和婴儿器官实施麻醉是重要的。脊髓丘脑束（spinothalamic tract， STT）是将伤害性信息传递到大脑的一个重要途径，然而脊髓细胞轴突到达丘脑的发育阶段尚不清楚。因此，逆行示踪剂氟金被用于表征小鼠发育的几个阶段的STT，这是一个以前未研究过STT的物种。1周龄、2日龄和胚胎期第18天的小鼠在逆行标记的STT神经元的数量和分布上与成年小鼠没有差异。在每个年龄组中，大约有3500个神经元从丘脑的一侧逆行标记。80%的标记细胞位于注射部位对侧的脊髓一侧。63%的标记细胞位于颈髓内，18%位于胸髓内，19%位于腰骶脊髓内。逆行标记的细胞直径在出生后一周内显著增加。将顺行示踪剂生物素化右旋糖酐胺注入新生儿脊髓后，观察到丘脑腹底复合体内的树突和突起。这些数据表明，虽然STT的神经元在出生后继续增大，但它们的轴突在出生前到达丘脑，并具有功能所需的一些形态特征。
Understanding the development of nociceptive circuits is important for the proper treatment of pain and administration of anesthesia to prenatal, newborn and infant organisms. The spinothalamic tract (STT) is an integral pathway in the transmission of nociceptive information to the brain, yet the stage of development when axons from cells in the spinal cord reach the thalamus is unknown. Therefore, the retrograde tracer Fluoro-Gold was used to characterize the STT at several stages of development in the mouse, a species in which the STT was previously unexamined. One week old, two day old and embryonic day 18 mice did not differ from adults in the number or distribution of retrogradely labeled STT neurons. Approximately 3,500 neurons were retrogradely labeled from one side of the thalamus in each age group. Eighty percent of the labeled cells were located on the side of the spinal cord contralateral to the injection site. Sixty-three percent of all labeled cells were located within the cervical cord, 18% in thoracic cord and 19% in the lumbosacral spinal cord. Retrogradely labeled cells significantly increased in diameter over the first postnatal week. Arborizations and boutons within the ventrobasal complex of the thalamus were observed after the anterograde tracer biotinylated dextran amine was injected into the neonatal spinal cord. These data indicate that while neurons of the STT continue to increase in size during the postnatal period, their axons reach the thalamus before birth and possess some of the morphological features required for functionality.
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DOI：
10.1152/ajpregu.2000.279.2.r560
发表时间：
2000-08-01
影响因子：
2.8
作者：
Chandler, MJ;Zhang, JH;Foreman, RD
通讯作者：
Foreman, RD
影响因子：
2.5
作者：
CRAIG, AD;LININGTON, AJ;KNIFFKI, KD
通讯作者：
KNIFFKI, KD
影响因子：
--
作者：
ABERCROMBIE, M
通讯作者：
ABERCROMBIE, M
影响因子：
2.5
作者：
Al-Khater, Khulood M.;Kerr, Robert;Todd, Andrew J.
通讯作者：
Todd, Andrew J.
影响因子：
5.5
作者：
Baccei, ML;Bardoni, R;Fitzgerald, M
通讯作者：
Fitzgerald, M