Optimal Pore Size of Honeycomb Polylactic Acid Films for In Vitro Cartilage Formation by Synovial Mesenchymal Stem Cells.
Optimal Pore Size of Honeycomb Polylactic Acid Films for In Vitro Cartilage Formation by Synovial Mesenchymal Stem Cells.
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DOI：
10.1155/2021/9239728
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发表时间：
2021
影响因子：
4.3
通讯作者：
Sekiya I
中科院分区：
文献类型：
--
作者：
Yagi M;Mizuno M;Fujisawa R;Katano H;Endo K;Ozeki N;Sakamaki Y;Koga H;Sekiya I
软骨组织工程需要选择合适的人工支架。聚乳酸（PLA）蜂窝膜由于其高孔隙率而被认为是高度可生物降解的和细胞粘附的。本研究的目的是确定蜂窝状PLA膜的最佳孔径，用于使用滑膜间充质干细胞（MSC）体外软骨形成。 将人滑膜间充质干细胞悬液接种在具有不同孔径（无孔或具有5 μm或20 μm孔）的PLA膜上，然后通过扫描电子显微镜观察。定量保留在膜中和穿过膜的细胞的数量。接种后一天，将培养基转换为软骨诱导培养基，并对膜进行延时成像和组织学观察。 5 μm孔膜显示MSC具有在几个孔之间延伸的伪足，而20 μm孔膜显示MSC体浸没在孔中。对于无孔的膜，粘附的MSC的数量显著较低，而对于20 μm孔的膜，穿过膜的MSC的数量显著较高。诱导形成软骨的MSC在一天后从无孔膜上剥落为片状。MSCs在5 μm孔膜上生长2周后形成的软骨较在20 μm孔膜上生长的软骨厚。 具有5 μm孔隙的蜂窝状PLA膜适合于滑膜MSCs体外软骨形成。
Tissue engineering of cartilage requires the selection of an appropriate artificial scaffold. Polylactic acid (PLA) honeycomb films are expected to be highly biodegradable and cell adhesive due to their high porosity. The purpose of this study was to determine the optimal pore size of honeycomb PLA films for in vitro cartilage formation using synovial mesenchymal stem cells (MSCs). Suspensions of human synovial MSCs were plated on PLA films with different pore sizes (no pores, or with 5 μm or 20 μm pores) and then observed by scanning electron microscopy. The numbers of cells remaining in the film and passing through the film were quantified. One day after plating, the medium was switched to chondrogenic induction medium, and the films were time-lapse imaged and observed histologically. The 5 μm pore film showed MSCs with pseudopodia that extended between several pores, while the 20 μm pore film showed MSC bodies submerged into the pores. The number of adhered MSCs was significantly lower for the film without pores, while the number of MSCs that passed through the film was significantly higher for the 20 μm pore film. MSCs that were induced to form cartilage peeled off as a sheet from the poreless film after one day. MSCs formed thicker cartilage at two weeks when growing on the 5 μm pore films than on the 20 μm pore films. Honeycomb PLA films with 5 μm pores were suitable for in vitro cartilage formation by synovial MSCs.
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影响因子：
3.9
作者：
Yamamoto, S.;Tanaka, M.;Shimomura, M.
通讯作者：
Shimomura, M.
影响因子：
2.3
作者：
Kohno Y;Mizuno M;Ozeki N;Katano H;Otabe K;Koga H;Matsumoto M;Kaneko H;Takazawa Y;Sekiya I
通讯作者：
Sekiya I
影响因子：
7.5
作者：
Mizuno M;Katano H;Mabuchi Y;Ogata Y;Ichinose S;Fujii S;Otabe K;Komori K;Ozeki N;Koga H;Tsuji K;Akazawa C;Muneta T;Sekiya I
通讯作者：
Sekiya I
影响因子：
7.5
作者：
Mizuno M;Katano H;Otabe K;Komori K;Kohno Y;Fujii S;Ozeki N;Horie M;Tsuji K;Koga H;Muneta T;Sekiya I
通讯作者：
Sekiya I
影响因子：
3.7
作者：
Olivares-Navarrete R;Lee EM;Smith K;Hyzy SL;Doroudi M;Williams JK;Gall K;Boyan BD;Schwartz Z
通讯作者：
Schwartz Z