Three-dimensional scaffolds of acellular human and porcine lungs for high throughput studies of lung disease and regeneration.
Three-dimensional scaffolds of acellular human and porcine lungs for high throughput studies of lung disease and regeneration.
复制标题
DOI：
10.1016/j.biomaterials.2013.11.078
复制
发表时间：
2014-03
期刊：
影响因子：
14
通讯作者：
Weiss, Daniel J.
中科院分区：
文献类型：
--
作者：
Wagner, Darcy E.;Bonenfant, Nicholas R.;Sokocevic, Dino;DeSarno, Michael J.;Borg, Zachary D.;Parsons, Charles S.;Brooks, Elice M.;Platz, Joseph J.;Khalpey, Zain I.;Hoganson, David M.;Deng, Bin;Lam, Ying W.;Oldinski, Rachael A.;Ashikaga, Takamaru;Weiss, Daniel J.
关键词：
来自复杂的整个器官如肺的脱细胞支架越来越多地被研究用于离体器官生成和用于细胞-细胞外基质相互作用的体外研究。我们已经建立了用于大动物和人肺的有效去细胞化和再细胞化的有效方法，包括允许切除保留三维肺结构的多个小段（101 - 3cm 3）的技术。结合使用合成胸膜涂层，可以通过保存的血管和气道导管选择性地生理接种细胞。接种的片段可以进一步切片用于高通量研究。此外，我们证明了热成像作为一种强大的非侵入性技术，用于监测灌注脱细胞化和评估人类和猪肺脱细胞化后血管和气道网络的保存。总的来说，这些技术是向前迈出的重要一步，因为它们允许在生物学相关性更高的三维无细胞支架中对单个肺或肺叶进行高通量体外研究。
Acellular scaffolds from complex whole organs such as lung are being increasingly studied for ex vivo organ generation and for in vitro studies of cell-extracellular matrix interactions. We have established effective methods for efficient de- and recellularization of large animal and human lungs including techniques which allow multiple small segments (∼1–3cm3) to be excised that retain 3-dimensional lung structure. Coupled with the use of a synthetic pleural coating, cells can be selectively physiologically inoculated via preserved vascular and airway conduits. Inoculated segments can be further sliced for high throughput studies. Further, we demonstrate thermography as a powerful noninvasive technique for monitoring perfusion decellularization and for evaluating preservation of vascular and airway networks following human and porcine lung decellularization. Collectively, these techniques are a significant step forward as they allow high throughput in vitro studies from a single lung or lobe in a more biologically relevant, three-dimensional acellular scaffold.
登录
查看更多内容
影响因子：
23.9
作者：
Longmire, Tyler A.;Ikonomou, Laertis;Hawkins, Finn;Christodoulou, Constantina;Cao, Yuxia;Jean, J. C.;Kwok, Letty W.;Mou, Hongmei;Rajagopal, Jayaraj;Shen, Steven S.;Dowton, Anne A.;Serra, Maria;Weiss, Daniel J.;Green, Michael D.;Snoeck, Hans-Willem;Ramirez, Maria I.;Kotton, Darrell N.
通讯作者：
Kotton, Darrell N.
影响因子：
3.9
作者：
Liu, Kan;Ding, Hui-Jiang;Zhao, Xing-Zhong
通讯作者：
Zhao, Xing-Zhong
影响因子：
82.9
作者：
Ott, Harald C.;Clippinger, Ben;Vacanti, Joseph P.
通讯作者：
Vacanti, Joseph P.
DOI：
10.1126/science.1189345
发表时间：
2010-07-30
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Petersen TH;Calle EA;Zhao L;Lee EJ;Gui L;Raredon MB;Gavrilov K;Yi T;Zhuang ZW;Breuer C;Herzog E;Niklason LE
通讯作者：
Niklason LE
影响因子：
4.1
作者：
Cortiella, Joaquin;Niles, Jean;Nichols, Joan E.
通讯作者：
Nichols, Joan E.