The ABA receptor-like gene VyPYL9 from drought-resistance wild grapevine confers drought tolerance and ABA hypersensitivity in Arabidopsis
The ABA receptor-like gene VyPYL9 from drought-resistance wild grapevine confers drought tolerance and ABA hypersensitivity in Arabidopsis
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来自抗旱野生葡萄的 ABA 受体样基因 VyPYL9 赋予拟南芥耐旱性和 ABA 超敏性
DOI：
10.1007/s11240-019-01650-2
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发表时间：
2019-08
期刊：
影响因子：
--
通讯作者：
Wen Ying-Qiang
中科院分区：
文献类型：
--
作者：
Liu Jie;Zhao Feng-Li;Guo Ye;Fan Xiu-cai;Wang Yue-jin;Wen Ying-Qiang
PYR/PYL/RCAR（以下简称PYL）蛋白作为脱落酸（ABA）受体，在干旱胁迫响应中起着至关重要的作用。在本研究中，我们以耐旱的野生中国葡萄葡萄品种“燕山1号”为模型，了解pyl在葡萄和其他水果作物中的功能。我们从叶山弧菌中克隆了6个PYL基因。系统发育分析表明，编码蛋白可分为3个亚科。所有六种葡萄藤PYL蛋白在ABA结合的“门-锁存”机制和与PP2C蛋白相互作用的位置上都含有保守的氨基酸。VyPYLs在‘燕山1号’植株各组织中的基因表达谱显示，VyPYLs在老叶中的表达相对较强。亚细胞定位分析表明，VyPYL蛋白定位于细胞质和细胞核。酵母双杂交实验显示，6个vyyl蛋白以aba依赖或aba独立的方式选择性地与3个VyPP2C蛋白相互作用。外源ABA和干旱条件诱导了其中一个基因VyPYL9的表达。VyPYL9在拟南芥中的异源表达增强了种子萌发和初生根生长过程中ABA的敏感性。此外，表达vypyl9的拟南芥植物表现出更强的耐旱性，这与减少水分流失和增加抗氧化防御系统的活性有关。此外，我们发现ABA促进了表达VyPYL9的拟南芥植株的叶片衰老。综上所述，我们的研究结果表明，VyPYL9可能通过ABA信号通路参与葡萄对干旱胁迫的响应。表达vypyl9的拟南芥植物表现出更强的耐旱性，这与减少水分流失和增加抗氧化防御系统的活性有关。此外，我们发现ABA促进了表达VyPYL9的拟南芥植株的叶片衰老。
The PYR/PYL/RCAR (hereafter referred to PYLs) proteins, which act as abscisic acid (ABA) receptors, have been reported to play a crucial role in response to drought stress. In this study, we used a drought-resistant accession of the wild Chinese grapevine, Vitis yeshanensis-’Yanshan-1’, as a model to understand the function of PYLs in grape and other fruit crops. We cloned six PYL genes from V. yeshanensis. Phylogenetic analysis showed that the encoded proteins could be classified into three subfamilies. All six grapevine PYL proteins contained conserved amino acids at positions implicated in ‘gate-and-latch’ mechanism of ABA binding and in the interaction with PP2C proteins. Gene expression profiles of VyPYLs in various tissues of ‘Yanshan-1’ plants revealed they were relatively strongly expressed in old leaves compared to young leaves. Analysis of the subcellular localization of the VyPYL proteins showed that they were localized in the cytoplasm and the nucleus. Yeast two-hybrid assays revealed that six VyPYL proteins selectively interacted with three VyPP2C proteins in an ABA-dependent or ABA-independent manner. Expression of one of the genes, VyPYL9, was induced by exogenous ABA and drought conditions. Heterologous expression of VyPYL9 in Arabidopsis enhanced ABA sensitivity during seed germination and primary root growth. Moreover, VyPYL9-expressing Arabidopsis plants showed enhanced drought tolerance, and this was associated with decreased water loss and increased activity of antioxidant defense systems. In addition, we found that ABA promoted leaf senescence in Arabidopsis plants expressing VyPYL9. Taken together, our results suggest that VyPYL9 may be involved in response of grapevine to drought stress via the ABA signaling pathway. VyPYL9-expressing Arabidopsis plants showed enhanced drought tolerance, and this was associated with decreased water loss and increased activity of antioxidant defense systems. In addition, we found that ABA promoted leaf senescence in Arabidopsis plants expressing VyPYL9.
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DOI：
10.1126/science.1173041
发表时间：
2009-05-22
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Park SY;Fung P;Nishimura N;Jensen DR;Fujii H;Zhao Y;Lumba S;Santiago J;Rodrigues A;Chow TF;Alfred SE;Bonetta D;Finkelstein R;Provart NJ;Desveaux D;Rodriguez PL;McCourt P;Zhu JK;Schroeder JI;Volkman BF;Cutler SR
通讯作者：
Cutler SR
ABA 受体亚家族 III 增强拟南芥脱落酸敏感性并提高耐旱性
影响因子：
5.6
作者：
Li X;Li G;Li Y;Kong X;Zhang L;Wang J;Li X;Yang Y
通讯作者：
Yang Y
影响因子：
27.5
作者：
Lee, Sung Chul;Lim, Chae Woo;Luan, Sheng
通讯作者：
Luan, Sheng
影响因子：
6.9
作者：
González-Guzmán M;Rodríguez L;Lorenzo-Orts L;Pons C;Sarrión-Perdigones A;Fernández MA;Peirats-Llobet M;Forment J;Moreno-Alvero M;Cutler SR;Albert A;Granell A;Rodríguez PL
通讯作者：
Rodríguez PL
影响因子：
3.7
作者：
Liang X;Garcia BL;Visai L;Prabhakaran S;Meenan NA;Potts JR;Humphries MJ;Höök M
通讯作者：
Höök M