Using green fluorescent malaria parasites to screen for permissive vector mosquitoes.
Using green fluorescent malaria parasites to screen for permissive vector mosquitoes.
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DOI：
10.1186/1475-2875-5-23
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发表时间：
2006-03-28
期刊：
影响因子：
3
通讯作者：
Menard R
中科院分区：
文献类型：
--
作者：
Frischknecht F;Martin B;Thiery I;Bourgouin C;Menard R
感染啮齿动物的疟原虫属，特别是伯氏疟原虫和约氏疟原虫，可用于研究宿主-寄生虫相互作用。在东南亚、非洲和南美洲作为人类疟原虫载体的蚊子物种对啮齿类疟原虫物种的感染表现出不同的易感性。伯氏疟原虫和约氏疟原虫感染冈比亚按蚊和斯氏按蚊，它们分别主要在非洲和亚洲发现。然而，据报道，约氏疟原虫可以感染南美白纹按蚊，而伯氏疟原虫不能。表达绿色荧光蛋白的伯氏疟原虫系用于筛选易受伯氏疟原虫感染的蚊子。检查并筛查活蚊子腹部是否存在荧光信号。感染的蚊子，然后通过延时显微镜检查，以揭示子孢子在血淋巴和唾液腺提取的动态行为。在活蚊体内可检测到单个荧光卵囊，伯氏疟原虫可感染A。albimanus。与其他蚊子一样，伯氏疟原虫子孢子可以漂浮在血淋巴中并侵入A.皮下注射后对小鼠具有感染性。荧光疟原虫可用于快速筛选易感蚊子。这些结果为在输入A. gambiae和A.史蒂芬西是不允许的。
The Plasmodium species that infect rodents, particularly Plasmodium berghei and Plasmodium yoelii, are useful to investigate host-parasite interactions. The mosquito species that act as vectors of human plasmodia in South East Asia, Africa and South America show different susceptibilities to infection by rodent Plasmodium species. P. berghei and P. yoelii infect both Anopheles gambiae and Anopheles stephensi, which are found mainly in Africa and Asia, respectively. However, it was reported that P. yoelii can infect the South American mosquito, Anopheles albimanus, while P. berghei cannot. P. berghei lines that express the green fluorescent protein were used to screen for mosquitoes that are susceptible to infection by P. berghei. Live mosquitoes were examined and screened for the presence of a fluorescent signal in the abdomen. Infected mosquitoes were then examined by time-lapse microscopy to reveal the dynamic behaviour of sporozoites in haemolymph and extracted salivary glands. A single fluorescent oocyst can be detected in live mosquitoes and P. berghei can infect A. albimanus. As in other mosquitoes, P. berghei sporozoites can float through the haemolymph and invade A. albimanus salivary glands and they are infectious in mice after subcutaneous injection. Fluorescent Plasmodium parasites can be used to rapidly screen susceptible mosquitoes. These results open the way to develop a laboratory model in countries where importation of A. gambiae and A. stephensi is not allowed.
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影响因子：
2.1
作者：
BEIER, MS;PUMPUNI, CB;DAVIS, JR
通讯作者：
DAVIS, JR
影响因子：
3.4
作者：
Natarajan, R;Thathy, V;Vernick, KD
通讯作者：
Vernick, KD
DOI：
10.4269/ajtmh.1996.54.214
发表时间：
1996-02-01
影响因子：
3.3
作者：
Pumpuni, CB;DeMaio, J;Beier, JC
通讯作者：
Beier, JC
影响因子：
1.3
作者：
VANDERBERG, JP
通讯作者：
VANDERBERG, JP
影响因子：
3.8
作者：
Siden-Kiamos, I;Louis, C
通讯作者：
Louis, C