Myricetin suppresses UVB-induced wrinkle formation and MMP-9 expression by inhibiting Raf.
Myricetin suppresses UVB-induced wrinkle formation and MMP-9 expression by inhibiting Raf.
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DOI：
10.1016/j.bcp.2010.01.004
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发表时间：
2010-05-15
影响因子：
5.8
通讯作者：
Lee, Hyong Joo
中科院分区：
文献类型：
--
作者：
Jung, Sung Keun;Lee, Ki Won;Kim, Ho Young;Oh, Mi Hyun;Byun, Sanguine;Lim, Sung Hwan;Heo, Yong-Seok;Kang, Nam Joo;Bode, Ann M.;Dong, Zigang;Lee, Hyong Joo
长期暴露于太阳紫外线（UV）光会导致皮肤光老化。许多研究表明，天然存在的植物化学物质具有抗光老化作用，但其直接靶分子和机制尚不清楚。我们发现，杨梅苷，浆果和红葡萄酒中的主要黄酮类化合物，抑制皱纹的形成，在小鼠皮肤诱导的慢性UVB照射（0.18 J/cm 2，3天/周，15周）。杨梅素治疗减少UVB诱导的小鼠皮肤表皮增厚，也抑制UVB诱导的基质金属蛋白酶-9（MMP-9）的蛋白表达和酶活性。杨梅素似乎通过抑制UVB诱导的Raf激酶活性和随后的UVB诱导的MEK和ERK磷酸化的衰减来发挥其抗衰老作用。在体外和体内的下拉实验表明，杨梅素结合Raf在ATP的非竞争性方式。总体而言，这些结果表明，杨梅素通过抑制Raf激酶活性调节MMP-9的表达发挥有效的抗光老化活性。
Chronic exposure to solar ultraviolet (UV) light causes skin photoaging. Many studies have shown that naturally occurring phytochemicals have anti-photoaging effects, but their direct target molecule(s) and mechanism(s) remain unclear. We found that myricetin, a major flavonoid in berries and red wine, inhibited wrinkle formation in mouse skin induced by chronic UVB irradiation (0.18 J/cm2, 3 days/wk for 15 wk). Myricetin treatment reduced UVB-induced epidermal thickening of mouse skin and also suppressed UVB-induced matrix metalloproteinase-9 (MMP-9) protein expression and enzyme activity. Myricetin appeared to exert its anti-aging effects by suppressing UVB-induced Raf kinase activity and subsequent attenuation of UVB-induced phosphorylation of MEK and ERK in mouse skin. In vitro and in vivo pull-down assays revealed that myricetin bound with Raf in an ATP-noncompetitive manner. Overall, these results indicate that myricetin exerts potent anti-photoaging activity by regulating MMP-9 expression through the suppression of Raf kinase activity.
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影响因子：
6.5
作者：
Kim, Hyeon Ho;Cho, Soyun;Chung, Jin Ho
通讯作者：
Chung, Jin Ho
影响因子：
6.5
作者：
GREWE, M;TREFZER, U;KRUTMANN, J
通讯作者：
KRUTMANN, J
影响因子：
7.5
作者：
BIRKEDALHANSEN, H
通讯作者：
BIRKEDALHANSEN, H
影响因子：
6.1
作者：
Castillo-Munoz, Noelia;Gomez-Alonso, Sergio;Hermosin-Gutierrez, Isidro
通讯作者：
Hermosin-Gutierrez, Isidro
影响因子：
3.3
作者：
CHATTERJEE, R;BENZINGER, MJ;BISSETT, DL
通讯作者：
BISSETT, DL