Effect of chitosan and carboxymethyl chitosan on fibrinogen structure and blood coagulation
Effect of chitosan and carboxymethyl chitosan on fibrinogen structure and blood coagulation
复制标题
壳聚糖和羧甲基壳聚糖对纤维蛋白原结构及凝血功能的影响
DOI：
10.1080/09205063.2013.777229
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发表时间：
2013-07
期刊：
影响因子：
--
通讯作者：
Xue, Wei
中科院分区：
文献类型：
--
作者：
Li, Nan;Wang, Qian;Liu, Zonghua;Xue, Wei
壳聚糖在组织工程支架、药物/基因传递系统、止血材料、抗菌材料、伤口敷料等生物医学领域有着广泛的应用。在这种情况下，壳聚糖与血液成分的相互作用是决定聚合物有效性和安全性的关键。本文研究了不同相对分子质量的壳聚糖及其衍生物羧甲基壳聚糖(CMC)对凝血相关蛋白结构和功能的影响。具体地说，利用紫外光谱、荧光光谱和圆二色谱研究了纤维蛋白原这一重要的凝血蛋白的结构和构象变化。通过活化部分凝血活酶时间(APTT)、凝血酶原时间(PT)、纤维蛋白原时间(FT)和血栓弹性图(TEG)检测，评价壳聚糖和CMC对凝血功能的影响。这些结果表明，壳聚糖与纤维蛋白原主要通过静电吸引形成络合物。因此，壳聚糖和CMC改变了纤维蛋白原的结构和构象。此外，壳聚糖和CMC的存在对APTT、PT和FT的影响不大，但通过改变TEG参数而导致凝血过程的显著异常。这些结果为研究壳聚糖和其他生物聚合物的生物响应提供了重要的分子基础。
Chitosan has numerous biomedical applications such as tissue engineering scaffolds, drug/gene delivery systems, hemostasis materials, antibacterial materials, wound dressing, etc. In any case, chitosan administered in vivo would positively or passively contact or enter blood tissue. In this situation, the interaction of chitosan with blood components is critical to determine the efficacy and safety of the polymer. In this study, the effect of chitosan with different molecular weight and its derivative carboxymethyl chitosan (CMC) on the structure and function of clotting-related proteins was studied. Specifically, the structural and conformational change of fibrinogen, an important clotting protein, was studied by using UV, fluorescence, and circular dichroism spectroscopy, respectively. Further, the impact of chitosan and CMC on the clotting function was evaluated with activated partial thromboplastin time (APTT), prothrombin time (PT), fibrinogen time (FT), and thromboelastography (TEG) assays. These results showed that, chitosan and fibrinogen can form complex mainly by electrostatic attraction. As a result, the structure and conformation of fibrinogen are altered by chitosan and CMC. Additionally, the presence of chitosan and CMC has little impact on the values of APTT, PT and FT, but causes significant abnormality in the clotting process by changing TEG parameters. These results provide important insight into the molecular basis for the biological response to chitosan and other biopolymers.
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影响因子：
9.9
作者：
Yongli;Xiu-fang;Yandao;Nanming;Tingying;Xinqi
通讯作者：
Yongli;Xiu-fang;Yandao;Nanming;Tingying;Xinqi
影响因子：
14
作者：
Meng, Sheng;Liu, Zongjun;Ge, Junbo
通讯作者：
Ge, Junbo
影响因子：
4.9
作者：
Fischer, Thomas H.;Bode, Arthur P.;Vournakis, John N.
通讯作者：
Vournakis, John N.
DOI：
10.1016/0278-2391(92)90194-5
发表时间：
1992
期刊：
Journal of oral and maxillofacial surgery : official journal of the American Association of Oral and Maxillofacial Surgeons
影响因子：
--
作者：
Perry R. Klokkevold;Paul Subar;Haruhisa Fukayama;Charles N. Bertolami
通讯作者：
Perry R. Klokkevold;Paul Subar;Haruhisa Fukayama;Charles N. Bertolami
影响因子：
6.2
作者：
Kainthan, RK;Janzen, J;Brooks, DE
通讯作者：
Brooks, DE