Activation of the Phenylpropanoid pathway in Nicotiana tabacum improves the performance of the whitefly Bemisia tabaci via reduced jasmonate signaling.
Activation of the Phenylpropanoid pathway in Nicotiana tabacum improves the performance of the whitefly Bemisia tabaci via reduced jasmonate signaling.
复制标题
DOI：
10.1371/journal.pone.0076619
复制
发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Morin S
中科院分区：
文献类型：
--
作者：
Alon M;Malka O;Eakteiman G;Elbaz M;Moyal Ben Zvi M;Vainstein A;Morin S
韧皮部取食昆虫可以操纵植物诱导的抗性，并能够通过诱导无效的水杨酸（SA）反应来抑制有效的茉莉酸/乙烯（JA/ET）防御。因此，转基因植物中苯丙素生物合成途径的激活预计会引起韧皮部取食昆虫与其寄主植物之间复杂的相互作用，因为预测了两种防御力量之间的矛盾：各种苯丙素的毒性和通过激活途径的分支积累SA。在此，我们研究了通过过度表达PAP1转录因子激活烟草中苯丙素途径对烟草粉虱（一种韧皮部摄食昆虫模型）的影响。我们的性能测试表明，尽管野生型（WT）植株积累的黄酮类化合物含量明显高于野生型（WT）植株，但过表达使转基因植株比野生型（WT）植株更适合烟粉虱的寄主。对SA （PR1a）和JA/ET （ERF1、COI1和AOC）通路的指示基因进行转录分析，并对SA和JA激素水平进行定量分析，结果表明，烟粉虱侵染期大于8小时，转基因植株的SA信号活性和WT植株的JA/ET信号活性均较高。综上所示，这些结果强调了JA/ et诱导的防御在保护植物免受烟粉虱和其他可能的韧皮部取食昆虫的侵袭中发挥的重要作用。这也表明了韧皮部取食者抑制这些防御以有效利用植物寄主的必要性。我们的数据还表明，苯丙素途径产生的防御化学物质对昆虫的适合度只有很小的影响。
Phloem-feeding insects can manipulate plant-induced resistance and are able to suppress effective jasmonic acid/ethylene (JA/ET) defenses by the induction of inefficient salicylic acid (SA) based responses. As a result, activation of the phenylpropanoid biosynthesis pathway in transgenic plants is anticipated to cause complex interactions between phloem-feeding insects and their host plants due to predicted contradiction between two defense forces: the toxicity of various phenylpropanoids and the accumulation of SA via a branch of the activated pathway. Here, we investigated the effect of activating the phenylpropanoids pathway in Nicotiana tabacum, by over-expression of the PAP1 transcription factor, on the whitefly Bemisia tabaci, a phloem-feeding insect model. Our performance assays indicated that the over-expression made the transgenic plants a more suitable host for B. tabaci than wild-type (WT) plants, although these plants accumulated significantly higher levels of flavonoids. Transcription analyses of indicator genes in the SA (PR1a) and JA/ET (ERF1, COI1 and AOC) pathways followed by quantification of the SA and JA hormone levels, indicated that B. tabaci infestation periods longer than 8 hours, caused higher levels of activity of SA signaling in transgenic plants and higher levels of JA/ET signaling in WT plants. Taken together, these results emphasize the important role JA/ET-induced defenses play in protecting plants from successful infestation by B. tabaci and likely other phloem-feeding insects. It also indicates the necessity of phloem feeders to suppress these defenses for efficient utilization of plant hosts. Our data also indicate that the defensive chemistry produced by the phenylpropanoids pathway has only a minor effect on the insect fitness.
登录
查看更多内容
影响因子：
5.3
作者：
Cui H;Zhang ST;Yang HJ;Ji H;Wang XJ
通讯作者：
Wang XJ
影响因子：
2.3
作者：
Baldwin, IT
通讯作者：
Baldwin, IT
影响因子：
6.1
作者：
Johnson, ET;Dowd, PF
通讯作者：
Dowd, PF
影响因子：
3.8
作者：
Alon, Michal;Elbaz, Moshe;Morin, Shai
通讯作者：
Morin, Shai
影响因子：
13.8
作者：
Ben Zvi, Michal Moyal;Florence, Negre-Zakharov;Vainstein, Alexander
通讯作者：
Vainstein, Alexander