Semi-Automated Training of Rat Ultrasonic Vocalizations.
Semi-Automated Training of Rat Ultrasonic Vocalizations.
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DOI：
10.3389/fnbeh.2022.826550
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发表时间：
2022
影响因子：
3
通讯作者：
Shembel AC
中科院分区：
文献类型：
--
作者：
Johnson AM;Lenell C;Severa E;Rudisch DM;Morrison RA;Shembel AC
大鼠产生超声波发声（USVs）同种通信。这些USV是用于研究各种疾病和病症中的行为和机制变化的有价值的生物标志物。先前的工作已经证明，操作性条件反射可以在多周内逐渐增加大鼠产生的USV数量。这种操作性条件反射范式是一个有用的模型，用于研究喉肌使用增加对USV声学特性和潜在的中枢和外周喉感觉运动机制的影响。以前的USV操作性条件反射研究依赖于人工训练来引出USV生产，这是时间和劳动密集型的，并且可以引入人类变异性。本文介绍了一种半自动化的方法，用于训练大鼠，以增加其USV生产率配对市售的操作性空调设备与超声波检测系统。USV训练需要三个基本组成部分：启发线索，行为检测和奖励，以加强所需的行为。通过半自动化训练范例，可以使用间接暴露于异性或嗅觉线索来引起USV产生。引发的USV然后由超声波声学系统自动检测，从而触发蔗糖颗粒奖励的释放。我们的研究结果表明，这种半自动化的程序产生了类似的增加USV生产的手动培训方法。通过USV检测和奖励管理的自动化，可以最小化人员配备要求、人为错误和受试者行为可变性，同时增加可扩展性和可再现性。这种自动化也可以带来更大的实验灵活性，使USV培训模式变得更加可定制，适用于更广泛的应用。这种半自动的USV行为训练模式通过提高数据收集的易用性、速度和质量来改进手动训练技术。
Rats produce ultrasonic vocalizations (USVs) for conspecific communication. These USVs are valuable biomarkers for studying behavioral and mechanistic changes in a variety of diseases and disorders. Previous work has demonstrated operant conditioning can progressively increase the number of USVs produced by rats over multiple weeks. This operant conditioning paradigm is a useful model for investigating the effects of increased laryngeal muscle use on USV acoustic characteristics and underlying central and peripheral laryngeal sensorimotor mechanisms. Previous USV operant conditioning studies relied on manual training to elicit USV productions, which is both time and labor intensive and can introduce human variability. This manuscript introduces a semi-automated method for training rats to increase their rate of USV production by pairing commercially available operant conditioning equipment with an ultrasonic detection system. USV training requires three basic components: elicitation cue, detection of the behavior, and a reward to reinforce the desired behavior. With the semi-automated training paradigm, indirect exposure to the opposite sex or an olfactory cue can be used to elicit USV production. The elicited USV is then automatically detected by the ultrasonic acoustic system, which consequently triggers the release of a sucrose pellet reward. Our results demonstrate this semi-automated procedure produces a similar increase in USV production as the manual training method. Through automation of USV detection and reward administration, staffing requirements, human error, and subject behavioral variability may be minimized while scalability and reproducibility are increased. This automation may also result in greater experimental flexibility, allowing USV training paradigms to become more customizable for a wider array of applications. This semi-automated USV behavioral training paradigm improves upon manual training techniques by increasing the ease, speed, and quality of data collection.
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影响因子：
2.9
作者：
Lenell C;Johnson AM
通讯作者：
Johnson AM
人声训练，左旋多巴和环境对帕金森氏病大鼠神经毒素模型超声声音的影响。
影响因子：
2.7
作者：
Kelm-Nelson CA;Brauer AF;Ciucci MR
通讯作者：
Ciucci MR
影响因子：
1.2
作者：
Johnson, Aaron M.;Doll, Emerald J.;Ciucci, Michelle R.
通讯作者：
Ciucci, Michelle R.
影响因子：
2.5
作者：
Brudzynski, Stefan M.
通讯作者：
Brudzynski, Stefan M.
DOI：
10.1093/gerona/glt044
发表时间：
2013-12-01
影响因子：
5.1
作者：
Johnson, Aaron M.;Ciucci, Michelle R.;Connor, Nadine P.
通讯作者：
Connor, Nadine P.