Primary metabolism is associated with the astaxanthin biosynthesis in the green algae Haematococcus pluvialis under light stress
Primary metabolism is associated with the astaxanthin biosynthesis in the green algae Haematococcus pluvialis under light stress
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光胁迫下绿藻雨生红球藻的初级代谢与虾青素生物合成有关
DOI：
10.1016/j.algal.2019.101768
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发表时间：
2020-03
期刊：
影响因子：
--
通讯作者：
Nianjun Xu
中科院分区：
文献类型：
--
作者：
Chaoyang Hu;D;an Cui;Xue Sun;Jianxin Shi;Nianjun Xu
雨生红球藻（Haematococcus pluvialis）是一种天然生物资源，在各种生物和非生物胁迫条件下，特别是强光胁迫下，积累了大量的虾青素，是一种重要的生物活性物质。然而，总体上对强光的代谢反应，特别是初级代谢与虾青素积累之间的关系仍然不清楚。本研究首次采用非靶向代谢组学方法对H.不同光照强度下雨生藻虾青素积累的研究结果表明，雨生藻在不同光照强度下积累虾青素的能力不同，其中鉴定出200多种低分子代谢产物。将代谢组学数据与先前的转录组学数据相结合，发现了几条代谢途径，包括棉子糖家族寡糖生物合成途径、GSH代谢途径、嘧啶和嘌呤代谢途径、TCA循环和磷脂，这些代谢途径对高光强下的虾青素生物合成至关重要，从而确定了从初级代谢到虾青素生物合成的代谢转变。外源应用藻类培养基中的核苷酸、碳水化合物和氨基酸等相关代谢产物证实了初级代谢与虾青素生物合成之间的代谢联系。我们的研究结果提供了一个坚实的代谢基础，操纵在强光胁迫下的虾青素生产，并揭示了代谢的见解，适应策略的H。pluvialisagainst光胁迫条件.
Haematococcus pluvialis is a kind of native bioresource, which accumulate high abundance of astaxanthin, one of the most important bioactive compounds, under various biotic and abiotic stress conditions particularly under high light. However, the global metabolic responses to high light in general and the relationship between primary metabolism and astaxanthin accumulation in particular are still obscure. In this study, non-targeted metabolomics approach was first to be used to profile the dynamic metabolic changes ofH. pluvialisalong astaxanthin accumulation under different light intensities, in which >200 low molecular metabolites were identified. The integration of metabolomics data with previous transcriptomic data uncovered several metabolic pathways including raffinose family oligosaccharides biosynthesis pathway, GSH metabolism pathways, pyrimidine and purine metabolism pathways, TCA cycle, and phospholipids, that are important for astaxanthin biosynthesis under high light intensity, pinpointing a metabolic shift from primary metabolism to astaxanthin biosynthesis. Exogenous application of related metabolites such as nucleotides, carbohydrates and amino acids in algal medium confirmed the metabolic link between primary metabolism and astaxanthin biosynthesis. Our results provides with a solid metabolic base for the manipulation of astaxanthin production under high light stress, and reveals metabolic insights into the adaption strategy ofH. pluvialisagainst light stress conditions.
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影响因子：
--
作者：
Chunhui Zhang;Jianguo Liu;Litao Zhang
通讯作者：
Litao Zhang
影响因子：
4.4
作者：
Sarada, R;Tripathi, U;Ravishankar, GA
通讯作者：
Ravishankar, GA
DOI：
10.1111/j.1365-3040.2012.02522.x
发表时间：
2012-10
期刊：
Plant, cell & environment
影响因子：
--
作者：
E. Zuther;Elisa Schulz;L. Childs;D. Hincha
通讯作者：
E. Zuther;Elisa Schulz;L. Childs;D. Hincha
影响因子：
2.1
作者：
Lemire, Joseph;Milandu, Yves;Appanna, Vasu D.
通讯作者：
Appanna, Vasu D.
影响因子：
11.4
作者：
Domínguez-Bocanegra, AR;Legarreta, IG;Campocosio, AT
通讯作者：
Campocosio, AT