Black phosphorus quantum dots nanocomposites based activatable bimodal imaging and determination of intracellular glutathione
Black phosphorus quantum dots nanocomposites based activatable bimodal imaging and determination of intracellular glutathione
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基于黑磷量子点纳米复合材料的可激活双峰成像和细胞内谷胱甘肽的测定
DOI：
10.1016/j.snb.2020.128518
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发表时间：
2020-10
期刊：
影响因子：
--
通讯作者：
Zhao Peng
中科院分区：
文献类型：
--
作者：
Li Hongmei;Xie Ruirui;Huang Cong;He Jiaqi;Yang Peipei;Tao Jia;Lin Bingquan;Zhao Peng
谷胱甘肽（GSH）与许多生理过程密切相关，GSH的灵敏测定对特定疾病的诊断和治疗具有重要意义。在这项工作中，提出了一种基于二氧化锰纳米片包裹黑磷量子点（BPQDs）纳米复合材料（BPQDs@MnO2）的可激活双峰成像和测定GSH的策略。加入GSH后，由于MnO2的减少，BPQDs的猝灭荧光可以恢复。在0.1 ~ 60 μM范围内，GSH的荧光可线性激活，检出限为35 nM （3σ/s）。由于mno2还原产生的Mn2+是一种良好的磁共振成像（MRI）造影剂，BPQDs@MnO2is应用于细胞内GSH的荧光/MRI双峰成像。MTT实验证明BPQDs@MnO2具有良好的生物相容性，并利用不同种类的细胞证明，用BPQDs@MnO2was孵育的细胞的双峰成像强度依赖于细胞内GSH的浓度。该策略为GSH的可活化性测定提供了一种新方法，并为活细胞GSH的荧光/MRI双峰成像提供了一个有前景的平台。
Glutathione (GSH) is closely related to many physiological processes, the sensitive determination of GSH is significant to the diagnostic and treatment of the specific diseases. In this work, an activatable bimodal imaging and determination strategy of GSH is proposed based on the nanocomposite of black phosphorus quantum dots (BPQDs) wrapped with manganese dioxide nanosheets (BPQDs@MnO2). The quenched fluorescence of BPQDs can be recovered with the addition of GSH, as a result of the reduction of MnO2. The BPQDs@MnO2appears a linearly activatable fluorescence to the addition of GSH from 0.1 μM to 60 μM with a limit of detection of 35 nM (3σ/s). As the Mn2+produced by the reduction of MnO2is a good magnetic resonance imaging (MRI) contrast agent, the BPQDs@MnO2is applied to the fluorescence/MRI bimodal imaging of intracellular GSH. MTT experiments prove the good biocompatibility of BPQDs@MnO2, and different kinds of cells are used to demonstrated that bimodal imaging intensity of cells incubated with BPQDs@MnO2was dependent on the concentration of intracellular GSH. This strategy provided a new method for the activatable determination of GSH, and offered a promising platform for fluorescence/MRI bimodal imaging of GSH in living cells.
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