Magnetic covalent organic framework nanospheres-based miRNA biosensor for sensitive glioma detection.
Magnetic covalent organic framework nanospheres-based miRNA biosensor for sensitive glioma detection.
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DOI：
10.1016/j.bioactmat.2021.11.033
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发表时间：
2022-08
影响因子：
18.9
通讯作者：
Huang R
中科院分区：
文献类型：
--
作者：
Liang D;Zhang X;Wang Y;Huo T;Qian M;Xie Y;Li W;Yu Y;Shi W;Liu Q;Zhu J;Luo C;Cao Z;Huang R
胶质瘤的敏感检测和准确诊断/预后仍然是迫切的挑战。本文采用单体介导的原位界面生长法制备了以均匀的Fe 3 O 4纳米组装体为核、高结晶度的磁性共价有机骨架纳米球（MCOF）。基于MCOF与发夹DNA的独特相互作用，构建了荧光信号放大的miRNA生物传感器。该方法可实现miRNA-182在不同基质中的灵敏检测，在真实的血样中的检测限、线性范围和决定系数（R2）分别达到20 fM、0.1 pM ~ 10 pM和0.991。此外，它具有良好的稳定性和精密度，从低的日内/日间RSD和高的提取回收率观察。结果表明，该方法可定量检测胶质瘤患者血清中的miRNA-182，其浓度明显高于健康人，且术后明显降低。最后，利用该生物传感器构建了一个概念验证的毛细管芯片系统，实现了微量样品中miRNA-182的可视化检测。这些发现为胶质瘤的敏感检测和准确诊断/预后提供了一种可靠的方法。采用原位界面生长法制备了尺寸均匀、结晶度高的磁性COF纳米球。基于MCOF介导的等温杂交链反应，研制了一种荧光信号放大的miRNA生物传感器。基于MCOF的生物传感器可以实现对不同基质中miRNA-182的灵敏检测。可以实现胶质瘤患者手术前后的准确诊断/预后。提出了一种概念验证的毛细管芯片，用于微量样品中miRNA-182的可视化检测。
Sensitive detection and accurate diagnosis/prognosis of glioma remain urgent challenges. Herein, dispersed magnetic covalent organic framework nanospheres (MCOF) with uniformed Fe3O4 nano-assembly as cores and high-crystalline COF as shells were prepared by monomer-mediated in-situ interface growth strategy. Based on the unique interaction between MCOF and hairpin DNA, a fluorescent signal amplified miRNA biosensor was constructed. It could realize the sensitive detection of miRNA-182 in different matrixes, where the detection limit, linearity range and determination coefficient (R2) in real blood samples reached 20 fM, 0.1 pM–10 pM and 0.991, respectively. Also, it possessed good stability and precision as observed from the low intra-day/inter-day RSD and high extraction recovery. As a result, it could quantify miRNA-182 in serum of glioma patients, the concentration of which was significantly higher than that of healthy people and obviously decreased after surgery. Finally, a proof-of-concept capillary chip system using this biosensor was proposed to realize the visualized detection of miRNA-182 in microsample. These findings suggest a robust way for sensitive detection and accurate diagnosis/prognosis of glioma. Magnetic COF nanospheres (MCOF) with uniformed size and high crystallinity were prepared by in-situ interface growth. A fluorescent signal amplified miRNA biosensor was developed based on the MCOF-mediated isothermal hybrid chain reaction. MCOF-based biosensor could achieve the sensitive miRNA-182 detection in different matrixes. Accurate diagnosis/prognosis of glioma patients before and after surgery could be achieved. A proof-of-concept capillary chip was proposed for the visualized detection of miRNA-182 in microsample.
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miR-182 作为神经胶质瘤进展和患者生存的预后标志物
影响因子：
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作者：
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通讯作者：
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作者：
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影响因子：
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作者：
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DOI：
10.1126/science.abb9601
发表时间：
2020-07-03
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Lennon AM;Buchanan AH;Kinde I;Warren A;Honushefsky A;Cohain AT;Ledbetter DH;Sanfilippo F;Sheridan K;Rosica D;Adonizio CS;Hwang HJ;Lahouel K;Cohen JD;Douville C;Patel AA;Hagmann LN;Rolston DD;Malani N;Zhou S;Bettegowda C;Diehl DL;Urban B;Still CD;Kann L;Woods JI;Salvati ZM;Vadakara J;Leeming R;Bhattacharya P;Walter C;Parker A;Lengauer C;Klein A;Tomasetti C;Fishman EK;Hruban RH;Kinzler KW;Vogelstein B;Papadopoulos N
通讯作者：
Papadopoulos N