Small non-coding RNA SraG regulates the operon YPK_1206-1205 in Yersinia pseudotuberculosis.

Small non-coding RNA SraG regulates the operon YPK_1206-1205 in Yersinia pseudotuberculosis.
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小非编码 RNA SraG 调节假结核耶尔森氏菌中的操纵子 YPK_1206-1205。

DOI:
10.1111/j.1574-6968.2012.02548.x
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发表时间:
2012-06
影响因子:
2.1
通讯作者:
张勇
张勇
中科院分区:
生物学4区
文献类型:
--
作者:
张勇

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小分子非编码RNA(Small non-coding RNAs,sRNA)是原核生物中重要的转录后调节因子,参与了许多细胞过程。SraG是在几种肠细菌物种中发现的sRNA,但其靶标尚未被表征。在这里,我们通过蛋白质组学分析比较了野生型和sraG缺失的假结核耶尔森菌突变体之间的蛋白质表达模式。16个蛋白质被上调或下调,并且证实了与YPK_1206-1205操纵子相关的SraG的负调控作用。进一步证明YPK_1206编码序列中的区域是这种负调控所需的。
Small non-coding RNAs (sRNAs) are important post-transcriptional regulators in prokaryotes and have been demonstrated to participate in most of the cellular processes. SraG is an sRNA found in several enterobacterial species, but its targets have not been characterized. Here, we compared the protein expression patterns between the wild-type and an sraG-depleted mutant of Yersinia pseudotuberculosis by proteomic analysis. Sixteen proteins were up- or downregulated, and the negative regulatory role of SraG associated with the YPK_1206-1205 operon was confirmed. A region in the coding sequence of YPK_1206 was further demonstrated to be required for this negative regulation.
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