Temperature-sensitive PSII: a novel approach for sustained photosynthetic hydrogen production.
Temperature-sensitive PSII: a novel approach for sustained photosynthetic hydrogen production.
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DOI：
10.1007/s11120-016-0232-3
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发表时间：
2016-12
影响因子：
3.7
通讯作者：
Nelson, Nathan
中科院分区：
文献类型：
--
作者：
Bayro-Kaiser, Vinzenz;Nelson, Nathan
关键词：
对能源的需求和相关的负担不断增长。至关重要的是开发新技术，为社会生产清洁和高效的能源，以避免即将到来的能源和环境危机。太阳光是地球上最丰富的能源。因此，它引起了我们的兴趣。某些微藻具有捕获太阳能并将其转移到能量载体H2的能力。氢气是一种有价值的燃料，因为它的燃烧只产生一种副产品：水。然而，有效的生物光解H2生产系统的建立受到三个主要障碍的阻碍：（1）放氢酶，[FeFe]-氢化酶，对氧高度敏感;（2）能量转换效率在经济上不可行;以及（3）产氢生物对大规模生产系统中的应激条件敏感。本研究的目的是规避与循环制氢系统，这一过程的氧敏感性。这种方法需要一种突变体，通过减少氧气释放来应对高温。为此，我们随机突变了绿色微藻莱茵衣藻，以产生表现出温度敏感性光合自养生长的突变体。所选择的突变体进一步通过它们在25和37 °C下放出氧和氢的能力来表征。我们为这个项目确定了四个候选突变体。我们对这些突变体进行了PSII荧光，P700吸光度和免疫印迹分析。最后，我们证明了这些突变体可以在原型产氢生物反应器中发挥作用。这些突变微藻代表了一种持续制氢的新方法。
The need for energy and the associated burden are ever growing. It is crucial to develop new technologies for generating clean and efficient energy for society to avoid upcoming energetic and environmental crises. Sunlight is the most abundant source of energy on the planet. Consequently, it has captured our interest. Certain microalgae possess the ability to capture solar energy and transfer it to the energy carrier, H2. H2 is a valuable fuel, because its combustion produces only one by-product: water. However, the establishment of an efficient biophotolytic H2 production system is hindered by three main obstacles: (1) the hydrogen-evolving enzyme, [FeFe]-hydrogenase, is highly sensitive to oxygen; (2) energy conversion efficiencies are not economically viable; and (3) hydrogen-producing organisms are sensitive to stressful conditions in large-scale production systems. This study aimed to circumvent the oxygen sensitivity of this process with a cyclic hydrogen production system. This approach required a mutant that responded to high temperatures by reducing oxygen evolution. To that end, we randomly mutagenized the green microalgae, Chlamydomonas reinhardtii, to generate mutants that exhibited temperature-sensitive photoautotrophic growth. The selected mutants were further characterized by their ability to evolve oxygen and hydrogen at 25 and 37 °C. We identified four candidate mutants for this project. We characterized these mutants with PSII fluorescence, P700 absorbance, and immunoblotting analyses. Finally, we demonstrated that these mutants could function in a prototype hydrogen-producing bioreactor. These mutant microalgae represent a novel approach for sustained hydrogen production.
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影响因子：
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通讯作者：
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