Co-localization of TRHR1 and LepRb receptors on neurons in the hindbrain of the rat.
Co-localization of TRHR1 and LepRb receptors on neurons in the hindbrain of the rat.
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DOI：
10.1016/j.brainres.2010.07.094
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发表时间：
2010-10-08
期刊：
影响因子：
2.9
通讯作者：
Hermann GE
中科院分区：
文献类型：
--
作者：
Barnes MJ;Rogers RC;Van Meter MJ;Hermann GE
我们已经报道了瘦素和促甲状腺激素释放激素（TRH）在后脑产生产热反应之间的高度合作的相互作用。在我们确定这种相互作用发生的机制之前，有必要确定瘦素和TRH协同作用增加产热的后脑部位。在这里，我们进行了热诱导的表位恢复技术和原位杂交，以确定是否神经元或传入纤维在后脑具有TRH 1型受体和长型瘦素受体[TRHR 1，LepRb，分别]。LepRb受体在孤束核（NST）、迷走神经背侧运动核（DMN）和延髓腹外侧区（VLM）的儿茶酚胺能神经元中有高表达。所有含有LepRb的神经元也含有TRHR 1。NST和中缝苍白[RP]和暗[RO]中具有LepRb受体的纤维在表型上被鉴定为多巴胺能2型纤维（vapor 2）。NST和RP中具有TRHR 1受体的纤维被表型鉴定为多巴胺能[即，5-羟色胺转运蛋白免疫阳性; SERT]。LepRb和TRHR 1的共同定位没有观察到单个纤维在后脑，但这两种纤维类型的混合在这些核。这些解剖结构的安排可能提供了一个基础，瘦素和TRH之间的协同作用，以增加产热。
We have reported a highly cooperative interaction between leptin and thyrotropin releasing hormone (TRH) in the hindbrain to generate thermogenic responses. Identifying the locus in the hindbrain where leptin and TRH act synergistically to increase thermogenesis will be necessary before we can determine the mechanism(s) by which this interaction occurs. Here, we performed heat-induced epitope recovery techniques and in situ hybridization to determine if neurons or afferent fibers in the hindbrain possess both TRH type 1 receptor and long-form leptin receptor [TRHR1; LepRb, respectively]. LepRb receptors were highly expressed in the solitary nucleus [NST], dorsal motor nucleus of the vagus [DMN] and catecholaminergic neurons of the ventrolateral medulla [VLM]. All neurons that contained LepRb also contained TRHR1. Fibers in the NST and the raphe pallidus [RP] and obscurrus [RO] that possess LepRb receptors were phenotypically identified as glutamatergic type 2 fibers (vglut2). Fibers in the NST and RP that possess TRHR1 receptors were phenotypically identified as serotonergic [i.e., immunopositive for the serotonin transporter; SERT]. Co-localization of LepRb and TRHR1 was not observed on individual fibers in the hindbrain but these two fiber types co-mingle in these nuclei. These anatomical arrangements may provide a basis for the synergy between leptin and TRH to increase thermogenesis.
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DOI：
10.1152/ajpregu.1999.276.2.r606
发表时间：
1999-02-01
影响因子：
2.8
作者：
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通讯作者：
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DOI：
10.1152/ajpregu.1998.275.5.r1627
发表时间：
1998-11-01
影响因子：
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影响因子：
2.8
作者：
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影响因子：
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作者：
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