Postthaw characterization of umbilical cord blood: markers of storage lesion.
Postthaw characterization of umbilical cord blood: markers of storage lesion.
复制标题
脐带血的后盘特征：储存病变的标记。
DOI：
10.1111/trf.12971
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发表时间：
2015-05
期刊：
影响因子：
2.9
通讯作者：
McKenna DH
中科院分区：
文献类型：
--
作者：
Hubel A;Spindler R;Curtsinger JM;Lindgren B;Wiederoder S;McKenna DH
脐带血（UCB）使用的持续增长将需要开发有意义的解冻后质量检测方法。本研究探讨了传统的和新的措施，用于评估UCB质量后，长期储存。研究的第一组涉及解冻短期（约1年）和长期（> 11年）储存的UCB。除了细胞凋亡外，还定量了常规解冻后测量（CFU、TNC、CD 34 +45+）。研究的第二组涉及将单位储存在液氮中，并通过将单位储存在-80 °C不同时间段来施加储存损伤。储存损伤后，解冻并评估这些装置。在研究的第一组中，短期和长期储存的UCB之间的解冻后测量值几乎没有差异。随着储存时间的延长，CD 34 +45+细胞的百分比略有增加，表达凋亡标志物的细胞数量减少。当从液氮转移到−80°C储存时，有核细胞计数变化不大，但CFU频率明显下降，表达早期和晚期凋亡标志物的细胞百分比增加。有核细胞计数不能反映长期储存期间造血祖细胞的损伤。半胱天冬酶和其他细胞凋亡标志物的表达提供了储存期间损伤的早期生物标志物，这与其他指标如CFU和CD 34 +45+细胞百分比一致。
The continued growth in the uses of umbilical cord blood (UCB) will require the development of meaningful post-thaw quality assays. This study examines both conventional and new measures for assessing UCB quality after long-term storage. The first arm of the study involved thawing UCB in storage for short (~1 year) and long period of time (> 11 years). Conventional post thaw measures (CFU, TNC, CD34+45+) were quantified in addition to apoptosis. The second arm of the study involved taking units stored in liquid nitrogen and imposing a storage lesion by storing the units in −80°C for various periods of time. After storage lesion, the units were thawed and assessed. In the first arm of the study, there was little difference in the post thaw measures between UCB stored for short and long periods of time. There was a slight increase in the percentage of CD34+45+ cells with time in storage and a reduction in the number of cells expressing apoptosis markers. When moved from liquid nitrogen to −80°C storage, the nucleated cell count varied little but there was a distinct drop in frequency of CFU and increase in percentage of cells expressing both early and late markers of apoptosis. Nucleated cell counts do not reflect damage to hematopoietic progenitors during long-term storage. Expression of caspases and other markers of apoptosis provide an early biomarker of damage during storage, which is consistent with other measures such as CFU and percentage of CD34+45+ cells.
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DOI：
10.1089/scd.1.1996.5.213
发表时间：
1996-06-01
期刊：
Journal of hematotherapy
影响因子：
--
作者：
Sutherland, D R;Anderson, L;Chin-Yee, I
通讯作者：
Chin-Yee, I
影响因子：
6.5
作者：
Shim, Jae-Seung;Cho, Bin;Oh, Il-Hoan
通讯作者：
Oh, Il-Hoan
影响因子：
2.1
作者：
Yamamoto, Shohei;Ikeda, Hirokazu;Isoyama, Keiichi
通讯作者：
Isoyama, Keiichi
影响因子：
158.5
作者：
GLUCKMAN, E;BROXMEYER, HE;BOYSE, EA
通讯作者：
BOYSE, EA
影响因子：
3.1
作者：
Butler, Merlin G.;Menitove, Jay E.
通讯作者：
Menitove, Jay E.