Mass Spectrometry Quantification, Localization, and Discovery of Feeding-Related Neuropeptides in Cancer borealis.
Mass Spectrometry Quantification, Localization, and Discovery of Feeding-Related Neuropeptides in Cancer borealis.
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DOI：
10.1021/acschemneuro.1c00007
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发表时间：
2021-02-17
影响因子：
5
通讯作者：
Li L
中科院分区：
文献类型：
--
作者：
DeLaney K;Hu M;Hellenbrand T;Dickinson PS;Nusbaum MP;Li L
螃蟹的巨蟹座神经系统是一个重要的模型，了解神经回路动力学及其调制，但在这个系统中的神经调节物质的身份和它们对回路动力学的影响仍然是不完整的，特别是在行为状态依赖的调制。因此，我们使用了多方面的质谱（MS）方法来识别区分未进食和进食状态的神经肽。双链稳定同位素标记显示，80的278个确定的神经肽的丰度是不同的神经节和/或神经血管组织从喂养与未喂养的动物。MS成像显示，另外7和11神经肽表现出改变的空间分布在大脑和神经内分泌心包器官（PO），分别在这两个喂养状态。此外，从头测序产生了69个新确定的推定的神经肽，可能会影响进食状态相关的神经调节。后两种神经肽在进食螃蟹的PO组织中被确定上调，并且这两种肽中的一种影响离体制剂的心跳。总的来说，这里提出的结果确定了一组神经肽，这些神经肽有望影响与进食相关的行为，为未来的功能研究提供了宝贵的机会。
The crab Cancer borealis nervous system is an important model for understanding neural circuit dynamics and its modulation, but the identity of neuromodulatory substances and their influence on circuit dynamics in this system remains incomplete, particularly with respect to behavioral state-dependent modulation. Therefore, we used a multifaceted mass spectrometry (MS) method to identify neuropeptides that differentiate the unfed and fed states. Duplex stable isotope labeling revealed that the abundance of 80 of 278 identified neuropeptides was distinct in ganglia and/or neurohemal tissue from fed vs. unfed animals. MS imaging revealed that an additional 7 and 11 neuropeptides exhibited altered spatial distributions in the brain and the neuroendocrine pericardial organs (POs), respectively, during these two feeding states. Furthermore, de novo sequencing yielded 69 newly identified putative neuropeptides that may influence feeding state-related neuromodulation. Two of these latter neuropeptides were determined to be upregulated in PO tissue from fed crabs and one of these two peptides influenced heartbeat in ex vivo preparations. Overall, the results presented here identify a cohort of neuropeptides that are poised to influence feeding-related behaviors, providing valuable opportunities for future functional studies.
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DOI：
10.1523/jneurosci.3079-09.2009
发表时间：
2009-09-30
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
DeLong ND;Kirby MS;Blitz DM;Nusbaum MP
通讯作者：
Nusbaum MP
温度变化对北极蟹影响的定量神经肽组学研究。
影响因子：
4.4
作者：
Chen, Ruibing;Xiao, Mingming;Buchberger, Amanda;Li, Lingjun
通讯作者：
Li, Lingjun
影响因子：
5.7
作者：
Blitz DM;Nusbaum MP
通讯作者：
Nusbaum MP
影响因子：
2.7
作者：
Dickinson, Patsy S.;Samuel, Heidi M.;Christie, Andrew E.
通讯作者：
Christie, Andrew E.
影响因子：
2.5
作者：
BOWMAN, JW;WINTERROWD, CA;GEARY, TG
通讯作者：
GEARY, TG