Nanomaterial cytotoxicity is composition, size, and cell type dependent.
Nanomaterial cytotoxicity is composition, size, and cell type dependent.
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DOI：
10.1186/1743-8977-7-22
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发表时间：
2010-08-21
影响因子：
10
通讯作者：
Tang L
中科院分区：
文献类型：
--
作者：
Sohaebuddin SK;Thevenot PT;Baker D;Eaton JW;Tang L
尽管进行了密集的研究工作，但关于细胞对纳米材料的反应的报道往往是不一致的，甚至是相互矛盾的。此外，响应细胞类型和纳米材料特性之间的关系还没有被很好地理解。我们利用三个代表不同生理室的模型细胞系和不同组成和大小的纳米材料，系统地研究了纳米材料的性质对细胞毒性程度和途径的影响。在本研究中，我们选择了不同组成的纳米材料(二氧化钛和二氧化硅纳米颗粒，以及不同尺寸的多壁碳纳米管(MWCNTs)，不同直径的纳米碳管(直径分别为8 nm、20-30 nm、50 nm；但长度为0.5-2μm))来分析组成和尺寸对3T3成纤维细胞、RAW 264.7巨噬细胞和端粒酶永生化的细支气管上皮细胞毒性的影响。在PBS和含血清溶液中表征纳米材料的特性后，细胞暴露在不同组成和大小的纳米材料中，通过降低线粒体的活性来监测细胞毒性。除了细胞毒性外，细胞对纳米材料的反应还包括定量产生活性氧物种、溶酶体膜失稳和线粒体通透性。然后分析这些反应对细胞命运--凋亡或坏死--的影响。纳米材料的毒性根据暴露的细胞类型和纳米材料的成分和大小而不同。此外，纳米材料暴露导致依赖于细胞类型的细胞内反应，导致每种纳米材料：细胞组合的细胞功能独特的崩溃。纳米材料诱导细胞特异性反应，导致不同的毒性和随后的细胞命运，基于暴露的细胞类型。我们的结果表明，纳米材料的组成和大小以及靶细胞类型是细胞内反应、细胞毒性程度和潜在毒性机制的关键决定因素。
Despite intensive research efforts, reports of cellular responses to nanomaterials are often inconsistent and even contradictory. Additionally, relationships between the responding cell type and nanomaterial properties are not well understood. Using three model cell lines representing different physiological compartments and nanomaterials of different compositions and sizes, we have systematically investigated the influence of nanomaterial properties on the degrees and pathways of cytotoxicity. In this study, we selected nanomaterials of different compositions (TiO2 and SiO2 nanoparticles, and multi-wall carbon nanotubes [MWCNTs]) with differing size (MWCNTs of different diameters < 8 nm, 20-30 nm, > 50 nm; but same length 0.5-2 μm) to analyze the effects of composition and size on toxicity to 3T3 fibroblasts, RAW 264.7 macrophages, and telomerase-immortalized (hT) bronchiolar epithelial cells. Following characterization of nanomaterial properties in PBS and serum containing solutions, cells were exposed to nanomaterials of differing compositions and sizes, with cytotoxicity monitored through reduction in mitochondrial activity. In addition to cytotoxicity, the cellular response to nanomaterials was characterized by quantifying generation of reactive oxygen species, lysosomal membrane destabilization and mitochondrial permeability. The effect of these responses on cellular fate - apoptosis or necrosis - was then analyzed. Nanomaterial toxicity was variable based on exposed cell type and dependent on nanomaterial composition and size. In addition, nanomaterial exposure led to cell type dependent intracellular responses resulting in unique breakdown of cellular functions for each nanomaterial: cell combination. Nanomaterials induce cell specific responses resulting in variable toxicity and subsequent cell fate based on the type of exposed cell. Our results indicate that the composition and size of nanomaterials as well as the target cell type are critical determinants of intracellular responses, degree of cytotoxicity and potential mechanisms of toxicity.
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影响因子：
3.8
作者：
Hirano, Seishiro;Kanno, Sanae;Furuyama, Akiko
通讯作者：
Furuyama, Akiko
影响因子：
4.5
作者：
Gurr, JR;Wang, ASS;Jan, KY
通讯作者：
Jan, KY
影响因子：
3.8
作者：
Clift, Martin J. D.;Rothen-Rutishauser, Barbara;Stone, Vicki
通讯作者：
Stone, Vicki
影响因子：
10.9
作者：
Brown, D. M.;Kinloch, I. A.;Stone, V.
通讯作者：
Stone, V.
影响因子：
4.1
作者：
Baird, SK;Kurz, T;Brunk, UT
通讯作者：
Brunk, UT