Potential of the Endophytic Fungus Phialocephala fortinii Rac56 Found in Rhodiola Plants to Produce Salidroside and p-Tyrosol.
Potential of the Endophytic Fungus Phialocephala fortinii Rac56 Found in Rhodiola Plants to Produce Salidroside and p-Tyrosol.
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DOI：
10.3390/molecules21040502
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发表时间：
2016-04-16
期刊：
影响因子：
--
通讯作者：
Wang J
中科院分区：
文献类型：
--
作者：
Cui J;Guo T;Chao J;Wang M;Wang J
2-（4-羟苯基）乙基-β-d-吡喃葡萄糖苷（红景天苷）和4-（2-羟乙基）苯酚（酪醇）是著名的食品和医药添加剂，最初来源于高山红景天属植物。通过UPLC/Q-TOF-MS和1H-NMR证实了内生真菌Rac 56（Phialocephala fortinii）产生红景天苷或酪醇。利用数据处理系统软件对发酵条件进行了优化。发酵结果表明，Rac 56的红景天苷和酪醇提取量稳定，分别为1.729 ± 0.06mg/mL和1.990 ± 0.05mg/mL。Rac 56发酵生产红景天苷和酪醇的最佳条件分别为：温度25 ℃，pH值7和5，装液量150 mL/250 mL三角瓶，转速100× g和200× g，发酵时间7和15 d。在此条件下，红景天苷和酪醇的产量分别为2.339 ± 0.1093 mg/mL和2.002 ± 0.0009 mg/mL，表明该菌株是一种很有前途的红景天苷和酪醇的来源。
2-(4-Hydroxyphenyl)ehyl-β-d-glucopyranoside (salidroside) and 4-(2-hydroxyethyl)phenol (p-tyrosol) are famous food and medicine additives originally derived from alpine Rhodiola plants. Salidroside or p-tyrosol production by the endophytic fungus Rac56 (Phialocephala fortinii) was confirmed by UPLC/Q-TOF-MS and 1H-NMR. The fermentation conditions were optimized by orthogonal design using data processing system software. The broth fermentation results showed that salidroside and p-tyrosol extraction yields from Rac56 were stable and reached 1.729 ± 0.06 mg and 1.990 ± 0.05 mg per mL of culture medium, respectively. The optimal conditions for salidroside and p-tyrosol production in fermentation culture of Rac56 were determined to be 25 °C, pH values of 7 and 5, Czapek-Dox culture medium volumes of 150 mL and 50 mL in 250 mL flasks, rotation speeds of 100× g and 200× g, and fermentation durations of 7 and 15 days, respectively. Under these optimal conditions, stable yields of 2.339 ± 0.1093 mg and 2.002 ± 0.0009 mg per mL of culture medium of salidroside and p-tyrosol, respectively, were obtained, indicating that the P. fortinii Rac56 strain is a promising source of these compounds.
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影响因子：
2.7
作者：
Shiono, Yoshihito;Shimanuki, Keiko;Kimura, Ken-ichi
通讯作者：
Kimura, Ken-ichi
影响因子：
3.3
作者：
Ioset, Karine Ndjoko;Nyberg, Nils T.;Jaroszewski, Jerzy W.
通讯作者：
Jaroszewski, Jerzy W.
影响因子：
3.8
作者：
Peschel, Wieland;Prieto, Jose M.;Williamson, Elizabeth M.
通讯作者：
Williamson, Elizabeth M.
DOI：
10.1139/b04-030
发表时间：
2004-05-01
期刊：
CANADIAN JOURNAL OF BOTANY-REVUE CANADIENNE DE BOTANIQUE
影响因子：
--
作者：
Wilson, BJ;Addy, HD;Currah, RS
通讯作者：
Currah, RS
DOI：
10.1002/nt.2620010306
发表时间：
1992-01-01
期刊：
Natural Toxins
影响因子：
--
作者：
Petrini, Orlando;Sieber, Thomas N.;Viret, Olivier
通讯作者：
Viret, Olivier