Characterization of a pathogenesis-related thaumatin-like protein gene TaPR5 from wheat induced by stripe rust fungus.
Characterization of a pathogenesis-related thaumatin-like protein gene TaPR5 from wheat induced by stripe rust fungus.
复制标题
条锈菌诱导的小麦发病机制相关索马甜蛋白基因 TaPR5 的表征。
DOI：
10.1111/j.1399-3054.2009.01338.x
复制
发表时间：
2010-05
影响因子：
6.4
通讯作者：
中科院分区：
文献类型：
--
作者：
病原相关蛋白（PR）是植物在各种生物和非生物胁迫下诱导产生的一类蛋白，在植物防御系统中发挥重要作用。PR 5家族的蛋白质，也称为奇异果甜蛋白样蛋白（TLPs），已在许多植物物种中检测到。在这项研究中，从小麦叶片（cv.）中分离并鉴定了一个新的PR 5基因，命名为TaPR 5。水原11）感染条锈病致病型CY 23（不亲和相互作用）。TaPR 5被预测为编码173个氨基酸的蛋白质，估计分子量为17.6 kDa，理论pI为4.64。TaPR 5的氨基酸序列与大麦等植物的PR 5和TLPs序列相似性较高，且在其氨基端含有一个信号肽。Southern杂交分析表明TaPR 5基因是由单拷贝基因编码的。定量实时聚合酶链反应（qRT-PCR）分析显示，TaPR 5转录本在不相容相互作用中被显着诱导和上调，而在相容相互作用中检测到相对低水平的转录本。TaPR 5也被植物激素（SA、JA和阿坝）和胁迫刺激（创伤、低温和高盐）诱导。使用洋葱表皮细胞的测定表明TaPR 5蛋白质在质外体中积累。免疫细胞化学方法表明，TaPR 5蛋白在非亲和互作小麦叶片细胞壁上的表达量明显高于亲和互作小麦。
Pathogenesis-related (PR) proteins, induced in plants in response to various biotic and abiotic stresses, have been assumed to play a role in plant defense system. Proteins of the PR5 family, also named thaumatin-like proteins (TLPs), have been detected in numerous plant species. In this research, a novel PR5 gene, designated as TaPR5, was isolated and characterized from wheat leaves (cv. Suwon 11) infected by the stripe rust pathotype CY23 (incompatible interaction) using the rapid amplification of cDNA ends (RACE). TaPR5 was predicted to encode a protein of 173 amino acids with an estimated molecular mass of 17.6 kDa and a theoretical pI of 4.64. The deduced amino acid sequence of TaPR5 showed a significant sequence similarity with PR5 and TLPs from barley and other plants and contained a putative signal peptide at the amino terminus. Southern blot analysis indicated that TaPR5 is coded by a single-copy gene. Quantitative real-time polymerase chain reaction (qRT-PCR) analyses revealed that TaPR5 transcript is significantly induced and upregulated in the incompatible interaction while in the compatible interaction a relative low level of the transcript was detected. TaPR5 was also induced by phytohormones (SA, JA and ABA) and stress stimuli (wounding, cold temperature and high salinity). Using an assay of onion epidermal cells indicated accumulation of TaPR5 protein in the apoplast. The immunocytochemical method showed that the TaPR5 protein was detected on cell walls of wheat leaves in the incompatible interaction at markedly higher labeling density compared with the compatible interaction.
登录
查看更多内容
影响因子：
6.9
作者：
Yee-Wai Chan;W. L. Tung;M. Griffith;K. Chow
通讯作者：
Yee-Wai Chan;W. L. Tung;M. Griffith;K. Chow
影响因子：
3.5
作者：
Anzlovar, S;Dalla Serra, M;Menestrina, G
通讯作者：
Menestrina, G
影响因子：
11.6
作者：
Shirasu, K;Nakajima, H;Lamb, C
通讯作者：
Lamb, C
影响因子：
7.4
作者：
J. Sock;R. Rohringer;Z. Kang
通讯作者：
J. Sock;R. Rohringer;Z. Kang
影响因子：
2.7
作者：
E. Reiss;C. Horstmann
通讯作者：
E. Reiss;C. Horstmann