Protective Effects of Fucoidan on Epithelial-Mesenchymal Transition of Retinal Pigment Epithelial Cells and Progression of Proliferative Vitreoretinopathy
Protective Effects of Fucoidan on Epithelial-Mesenchymal Transition of Retinal Pigment Epithelial Cells and Progression of Proliferative Vitreoretinopathy
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岩藻依聚糖对视网膜色素上皮细胞上皮-间质转化和增殖性玻璃体视网膜病变进展的保护作用
DOI：
10.1159/000489246
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发表时间：
2018-04
影响因子：
--
通讯作者：
Wang Fang
中科院分区：
文献类型：
--
作者：
Zhang Yao;Zhao Dongwan;Yang Shuai;Yao Haipei;Li Min;Zhao Chun;Zhang Jingfa;Xu Guo-Tong;Li Hui;Wang Fang
背景/目的：澄清性玻璃体视网膜病变（PVR）是孔源性视网膜脱离的一种严重致盲并发症。视网膜色素上皮（RPE）细胞的上皮间质转化（EMT）被认为在PVR的发病机制中起关键作用。褐藻糖胶是一种海洋提取物，具有抗炎、抗氧化、抗癌等多种组织器官功能。在这项研究中，我们研究了褐藻糖胶对RPE细胞EMT的潜在作用及其对PVR发展的影响。研究方法：MTS、Transwell和胶原凝胶收缩试验分别用于测量RPE细胞的活力、迁移和收缩。mRNA和蛋白质表达分别通过实时定量PCR和蛋白质印迹分析进行评估。在体内，建立了色素兔PVR模型，以检测褐藻糖胶的抗PVR作用。结果如下：褐藻糖胶可逆转转化生长因子β1诱导的RPE细胞EMT，包括增加α-平滑肌肌动蛋白（α-SMA）和纤连蛋白的表达，下调E-钙粘蛋白的表达。此外，褐藻糖胶可抑制TGF-β1诱导的Smad 2/3磷酸化水平的上调。褐藻糖胶还能抑制TGF-β1诱导的RPE细胞的迁移和收缩。在体内，褐藻糖胶抑制兔眼实验性PVR的进展。组织学结果显示，褐藻糖胶抑制α-SMA阳性视网膜前膜的形成。结论：岩藻依聚糖对RPE细胞EMT和实验性PVR的保护作用提示岩藻依聚糖作为抗PVR剂具有潜在的临床应用价值。
Background/Aims: Proliferative vitreoretinopathy (PVR) is a severe blinding complication of rhegmatogenous retinal detachment. Epithelial-mesenchymal transition (EMT) of retinal pigment epithelial (RPE) cells is thought to play a pivotal role in the pathogenesis of PVR. Fucoidan, a marine extract, reportedly has many benefits effects in a variety of tissues and organs such as anti-inflammation, anti-oxidative stress, and anti-carcinogenesis. In this study, we investigated the potential role of fucoidan on EMT in RPE cells and its effect on the development of PVR. Methods: MTS, Transwell, and collagen gel contraction assays were employed to measure the viability, migration, and contraction of RPE cells, respectively. mRNA and protein expression were evaluated via real-time quantitative PCR and western blot analysis, respectively. In vivo, a pigmented rabbit model of PVR was established to examine the anti-PVR effect of fucoidan. Results: Fucoidan reversed the transforming growth factor (TGF)-β1-induced EMT of RPE cells, including the increased expression of α-smooth muscle actin (α-SMA) and fibronectin and down-regulation of E-cadherin in human primary RPE cells. Moreover, the upregulation of phosphorylated Smad2/3 induced by TGF-β1 was suppressed by fucoidan. Fucoidan also inhibited the migration and contraction of RPE cells induced by TGF-β1. In vivo, fucoidan inhibited the progression of experimental PVR in rabbit eyes. Histological findings showed that fucoidan suppressed the formation of α-SMA-positive epiretinal membranes. Conclusion: Our findings regarding the protective effects of fucoidan on the EMT of RPE cells and experimental PVR suggest the potential clinical application of fucoidan as an anti-PVR agent.
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猫实验性牵引性视网膜脱离。
DOI：
10.1007/bf00203323
发表时间：
1991
期刊：
Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie
影响因子：
--
作者：
Wilson,CA;Khawly,JA;Hatchell,DL;Machemer,R
通讯作者：
Machemer,R
骨形态发生蛋白4对视网膜色素上皮-间质转化的抑制作用
影响因子：
4.6
作者：
Yao H;Li H;Yang S;Li M;Zhao C;Zhang J;Xu G;Wang F
通讯作者：
Wang F
影响因子：
3.4
作者：
Soler, MVC;Gallo, JE;Suburo, AM
通讯作者：
Suburo, AM
影响因子：
4.4
作者：
Tamiya, Shigeo;Liu, LanHsin;Kaplan, Henry J.
通讯作者：
Kaplan, Henry J.
DOI：
10.1016/s0896-1549(01)00008-6
发表时间：
2002-03
期刊：
Ophthalmology clinics of North America
影响因子：
--
作者：
Ivana K. Kim;J. Arroyo
通讯作者：
Ivana K. Kim;J. Arroyo