Magnetic resonance microscopy of human and porcine neurons and cellular processes.
Magnetic resonance microscopy of human and porcine neurons and cellular processes.
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DOI：
10.1016/j.neuroimage.2012.01.050
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发表时间：
2012-04-02
期刊：
影响因子：
5.7
通讯作者：
Blackband, Stephen J.
中科院分区：
文献类型：
--
作者：
Flint, Jeremy J.;Hansen, Brian;Portnoy, Sharon;Lee, Choong-Heon;King, Michael A.;Fey, Michael;Vincent, Franck;Stanisz, Greg J.;Vestergaard-Poulsen, Peter;Blackband, Stephen J.
磁共振成像（MRI）凭借其无与伦比的安全生成软组织高对比度图像的能力，一直处于诊断临床医学的最前沿。不幸的是，由于分辨率的限制，临床扫描是最有用的检测与少数病理相关的宏观结构变化。此外，由于长期无法直接观察细胞水平的磁共振（MR）信号行为，这种信息的特征很差，通常必须推断。随着1986年MR显微镜的出现，人们能够测量迄今为止不可观察的组织结构的MR信号特性。最近，硬件技术的进一步改进使得可视化哺乳动物细胞结构的能力成为可能。在目前的研究中，我们通过对人类和猪α运动神经元的神经元细胞体和过程进行成像来扩展以前的工作。在猪组织中进行了补充成像研究，以证明与人类样本的定性相似性。此外，在猪α运动神经元细胞体及其最大突起部分（平均值= 1.7±0.5 μm2/ms，基于53个像素）以及包含细胞外间隙、微血管和神经元混合物的区域（0.59±0.37 μm2/ms，基于33个像素）内生成表观扩散系数（ADC）图。含α运动神经元的MR图像的三维重建显示了相邻细胞之间神经元投射的空间排列。成像中的这种进步预示着基于对包括功能组织的组分的直接观察和测量来构建MR信号行为的精确模型的能力。这些工具不仅有助于改善我们对临床上进行的宏观MRI的解释，而且它们可能用于开发新的鉴别诊断方法，以帮助早期检测多种神经病变。
With its unparalleled ability to safely generate high-contrast images of soft tissues, magnetic resonance imaging (MRI) has remained at the forefront of diagnostic clinical medicine. Unfortunately due to resolution limitations, clinical scans are most useful for detecting macroscopic structural changes associated with a small number of pathologies. Moreover, due to a longstanding inability to directly observe magnetic resonance (MR) signal behavior at the cellular level, such information is poorly characterized and generally must be inferred. With the advent of the MR microscope in 1986 came the ability to measure MR signal properties of theretofore unobservable tissue structures. Recently, further improvements in hardware technology have made possible the ability to visualize mammalian cellular structure. In the current study, we expand upon previous work by imaging the neuronal cell bodies and processes of human and porcine α-motor neurons. Complimentary imaging studies are conducted in pig tissue in order to demonstrate qualitative similarities to human samples. Also, apparent diffusion coefficient (ADC) maps were generated inside porcine α-motor neuron cell bodies and portions of their largest processes (mean = 1.7±0.5 μm2/ms based on 53 pixels) as well as in areas containing a mixture of extracellular space, microvasculature, and neuropil (0.59±0.37 μm2/ms based on 33 pixels). Three-dimensional reconstruction of MR images containing α-motor neurons shows the spatial arrangement of neuronal projections between adjacent cells. Such advancements in imaging portend the ability to construct accurate models of MR signal behavior based on direct observation and measurement of the components which comprise functional tissues. These tools would not only be useful for improving our interpretation of macroscopic MRI performed in the clinic, but they could potentially be used to develop new methods of differential diagnosis to aid in the early detection of a multitude of neuropathologies.
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影响因子：
64.8
作者：
AGUAYO, JB;BLACKBAND, SJ;HINTERMANN, M
通讯作者：
HINTERMANN, M
DOI：
10.1152/ajpcell.1996.271.6.c1895
发表时间：
1996-12-01
影响因子：
5.5
作者：
Hsu, EW;Aiken, NR;Blackband, SJ
通讯作者：
Blackband, SJ
影响因子：
3.3
作者：
Hsu, EW;Aiken, NR;Blackband, SJ
通讯作者：
Blackband, SJ
影响因子：
6.1
作者：
Badilita, Vlad;Kratt, Kai;Wallrabe, Ulrike
通讯作者：
Wallrabe, Ulrike
影响因子：
64.8
作者：
LAUTERBUR, PC
通讯作者：
LAUTERBUR, PC