Loss-of-function mutations affecting a specific Glycine max R2R3 MYB transcription factor result in brown hilum and brown seed coats.
Loss-of-function mutations affecting a specific Glycine max R2R3 MYB transcription factor result in brown hilum and brown seed coats.
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DOI：
10.1186/1471-2229-11-155
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发表时间：
2011-11-09
影响因子：
5.3
通讯作者：
Bilyeu K
中科院分区：
文献类型：
--
作者：
Gillman JD;Tetlow A;Lee JD;Shannon JG;Bilyeu K
虽然现代大豆品种以黄色种皮为特征，唯一的颜色变异是在Hila发现的，但祖先的条件是黑色种皮。种皮和HILA的着色都是由于苯丙烷途径的衍生物的存在，主要是花青素。20世纪20年代，随着孟德尔工作的重新发现，在遗传学的复兴时期，人们首次研究了大豆种皮和种脐着色的遗传学。尽管在过去的20年里，这种表型标记已被纳入大豆广泛的遗传图谱中，但棕色种皮现象(R基因座)背后的遗传基础至今仍未确定。为了确定控制R基因效应的基因(棕色种脐或种皮颜色)，我们利用批量分离分析，从一个分离了R基因座两个表型不同等位基因的群体中鉴定了重组系。通过使用一组新颖的、生物信息确定的简单序列重复(SSR)标记，尽管只使用了100个F6系的作图群体，但精细定位得到了加速，使我们能够将包含r基因的基因组区域限制在200 kbp以下。候选基因分析表明，一个影响种皮特异表达的R2R3MYB转录因子基因(Glyma09g36990)的功能缺失突变是棕色种脐表型的有力候选基因。我们观察到功能R基因候选基因的mRNA表达水平与负责花青素生物合成最后一步的UDP-葡萄糖：类黄酮3-O-葡萄糖基转移酶(UF3GT)基因之间几乎完全相关。相反，当表达Glyma09g36990零等位基因时，没有发现UF3GT基因上调。我们发现了影响我们的R基因座候选基因的四个功能缺失突变的等位基因系列。在广泛分布的大豆品种调查中，除了上位性显性I等位基因或灰色短柔毛的存在外，这些突变中的任何一个的存在都与棕色种皮/种脐表型完全相关，这两个等位基因都可以掩盖r等位基因的影响，导致黄色或浅黄色Hila。这些发现有力地表明，一个特定的种皮表达的R2R3MYB基因功能的丧失是导致大豆棕种皮/种脐表型的原因。
Although modern soybean cultivars feature yellow seed coats, with the only color variation found at the hila, the ancestral condition is black seed coats. Both seed coat and hila coloration are due to the presence of phenylpropanoid pathway derivatives, principally anthocyanins. The genetics of soybean seed coat and hilum coloration were first investigated during the resurgence of genetics during the 1920s, following the rediscovery of Mendel's work. Despite the inclusion of this phenotypic marker into the extensive genetic maps developed for soybean over the last twenty years, the genetic basis behind the phenomenon of brown seed coats (the R locus) has remained undetermined until now. In order to identify the gene responsible for the r gene effect (brown hilum or seed coat color), we utilized bulk segregant analysis and identified recombinant lines derived from a population segregating for two phenotypically distinct alleles of the R locus. Fine mapping was accelerated through use of a novel, bioinformatically determined set of Simple Sequence Repeat (SSR) markers which allowed us to delimit the genomic region containing the r gene to less than 200 kbp, despite the use of a mapping population of only 100 F6 lines. Candidate gene analysis identified a loss of function mutation affecting a seed coat-specific expressed R2R3 MYB transcription factor gene (Glyma09g36990) as a strong candidate for the brown hilum phenotype. We observed a near perfect correlation between the mRNA expression levels of the functional R gene candidate and an UDP-glucose:flavonoid 3-O-glucosyltransferase (UF3GT) gene, which is responsible for the final step in anthocyanin biosynthesis. In contrast, when a null allele of Glyma09g36990 is expressed no upregulation of the UF3GT gene was found. We discovered an allelic series of four loss of function mutations affecting our R locus gene candidate. The presence of any one of these mutations was perfectly correlated with the brown seed coat/hilum phenotype in a broadly distributed survey of soybean cultivars, barring the presence of the epistatic dominant I allele or gray pubescence, both of which can mask the effect of the r allele, resulting in yellow or buff hila. These findings strongly suggest that loss of function for one particular seed coat-expressed R2R3 MYB gene is responsible for the brown seed coat/hilum phenotype in soybean.
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DOI：
10.1111/j.1365-313x.2006.02964.x
发表时间：
2007-02
期刊：
The Plant journal : for cell and molecular biology
影响因子：
--
作者：
Espley RV;Hellens RP;Putterill J;Stevenson DE;Kutty-Amma S;Allan AC
通讯作者：
Allan AC
影响因子：
5.3
作者：
Bolon YT;Joseph B;Cannon SB;Graham MA;Diers BW;Farmer AD;May GD;Muehlbauer GJ;Specht JE;Tu ZJ;Weeks N;Xu WW;Shoemaker RC;Vance CP
通讯作者：
Vance CP
影响因子：
2.6
作者：
HYMOWITZ, T
通讯作者：
HYMOWITZ, T
影响因子：
3.7
作者：
Hellens, Roger P.;Moreau, Carol;Ellis, T. H. Noel
通讯作者：
Ellis, T. H. Noel
影响因子：
5.4
作者：
LARK, KG;WEISEMANN, JM;MACALMA, T
通讯作者：
MACALMA, T