Simultaneous preparation and characterization of three high-purity type B fumonisins from maize culture
Simultaneous preparation and characterization of three high-purity type B fumonisins from maize culture
复制标题
玉米培养物中三种高纯度 B 型伏马菌素的同时制备和表征
DOI：
10.1039/c5ay03307a
复制
发表时间：
2016-03
影响因子：
3.1
通讯作者：
Zhihui Zhao
中科院分区：
文献类型：
--
作者：
Zheng Han;Junhua Yang;Qinxiong Rao;Zhihui Zhao
本研究建立了一种可靠的大规模制备方法，可从接种念珠镰刀菌的玉米培养基中同时制备高纯度伏马菌素B1 （FB1）、伏马菌素B2 （FB2）和伏马菌素B3 （FB3）。先用乙腈/水（50:50,v/v）提取b系列伏马菌素（FBs），然后用MAX滤芯纯化。在uni C18色谱柱（250 mm × 10 mm, 5 μm）和SB-CN色谱柱（250 mm × 9.4 mm, 5 μm）上进行色谱制备，有效消除其他干扰，获得高纯度化合物。经液相色谱-串联质谱（LC-MS/MS）和核磁共振（NMR）鉴定，化合物分别为FB1、FB2和FB3。FB1、FB2和FB3的纯度分别为96.25±0.18%、98.07±0.18%和97.36±0.74%，采用外标LC-MS/MS法测定。在72 h内，可以同时制备大量高纯度FB1 （80 mg）、FB2 （16 mg）和FB3 （16 mg）。
A reliable and large-scale preparative method was developed for the simultaneous preparation of high-purity fumonisin B1 (FB1), fumonisin B2 (FB2) and fumonisin B3 (FB3) from maize culture inoculated with Fusarium moniliforme in this study. The targeted B-series fumonisins (FBs) were firstly extracted with acetonitrile/water (50 : 50, v/v) and then purified using MAX cartridges. The subsequent chromatographic preparative procedures on a Unitary C18 column (250 mm × 10 mm, 5 μm) and a SB-CN column (250 mm × 9.4 mm, 5 μm) were performed to effectively eliminate the other interferents to obtain the high-purity compounds. The prepared compounds were clearly identified to be FB1, FB2 and FB3 using liquid chromatography-tandem mass spectrometry (LC-MS/MS) and nuclear magnetic resonance (NMR). The purities were 96.25 ± 0.18%, 98.07 ± 0.18% and 97.36 ± 0.74% for FB1, FB2 and FB3, respectively, which were determined by the external standard LC-MS/MS method. Within 72 h, large amounts of high-purity FB1 (80 mg), FB2 (16 mg) and FB3 (16 mg) could be simultaneously prepared by the established approach.
登录
查看更多内容
影响因子：
6.1
作者：
F. Meredith;C. Bacon;W. P. Norred;R. Plattner
通讯作者：
F. Meredith;C. Bacon;W. P. Norred;R. Plattner
影响因子：
1.9
作者：
Juan Carlos Angulo García;C. M. Ramos;P. Pinton;S. Elvira;I. Oswald
通讯作者：
Juan Carlos Angulo García;C. M. Ramos;P. Pinton;S. Elvira;I. Oswald
影响因子：
4.2
作者：
RILEY, RT;HINTON, DM;VOSS, KA
通讯作者：
VOSS, KA
影响因子：
3.1
作者：
Han, Zheng;Ren, Yiping;Wu, Yongjiang
通讯作者：
Wu, Yongjiang
影响因子：
1.6
作者：
A. Visconti;M. Solfrizzo;A. De Girolamo
通讯作者：
A. Visconti;M. Solfrizzo;A. De Girolamo