Facilitated diffusion of acetonitrile revealed by quantitative breath analysis using extractive electrospray ionization mass spectrometry.
Facilitated diffusion of acetonitrile revealed by quantitative breath analysis using extractive electrospray ionization mass spectrometry.
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使用萃取电喷雾电离质谱进行定量呼吸分析揭示了乙腈的促进扩散
DOI：
10.1038/srep01205
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发表时间：
2013
影响因子：
4.6
通讯作者：
Li, Hongmei
中科院分区：
文献类型：
--
作者：
Li, Ming;Ding, Jianhua;Gu, Haiwei;Zhang, Yan;Pan, Susu;Xu, Ning;Chen, Huanwen;Li, Hongmei
采用反应萃取电喷雾质谱法（EESI-MS），以银离子（Ag+）为离子试剂，在不进行任何前处理的情况下，对主动吸烟者、被动吸烟者和非吸烟者呼出气体样本中的乙腈浓度进行了体内定量测定。质谱联用中乙腈信号的检出限和相对标准偏差分别为0.16 ng/L和3.5% （n = 8）。有趣的是，在吸烟者戒烟后1 ~ 4小时内，人体呼吸中乙腈的浓度持续上升，7天后缓慢下降到本底水平。大量（> 165）样品的实验数据表明，吸入的乙腈很可能是通过促进扩散排出的，而不是之前报道的其他挥发性化合物的简单扩散。
By using silver cations (Ag+) as the ionic reagent in reactive extractive electrospray ionization mass spectrometry (EESI-MS), the concentrations of acetonitrile in exhaled breath samples from the volunteers including active smokers, passive smokers and non-smokers were quantitatively measuredin vivo, without any sample pretreatment. A limit of detection (LOD) and relative standard deviation (RSD) were 0.16 ng/L and 3.5% (n = 8), respectively, for the acetonitrile signals in MS/MS experiments. Interestingly, the concentrations of acetonitrile in human breath continuously increased for 1–4 hours after the smoker finished smoking and then slowly decreased to the background level in 7 days. The experimental data of a large number of (> 165) samples indicated that the inhaled acetonitrile is excreted most likely by facilitated diffusion, instead of simple diffusion reported previously for other volatile compounds.
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