Enhanced osteocalcin expression by osteoblast-like cells (MC3T3-E1) exposed to bioactive coating glass (SiO2-CaO-P2O5-MgO-K2O-Na2O system) ions.
Enhanced osteocalcin expression by osteoblast-like cells (MC3T3-E1) exposed to bioactive coating glass (SiO2-CaO-P2O5-MgO-K2O-Na2O system) ions.
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DOI：
10.1016/j.actbio.2009.05.035
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发表时间：
2009-11
影响因子：
9.7
通讯作者：
Marshall GW
中科院分区：
文献类型：
--
作者：
Varanasi VG;Saiz E;Loomer PM;Ancheta B;Uritani N;Ho SP;Tomsia AP;Marshall SJ;Marshall GW
本研究检验了用于植入物涂层的生物活性玻璃增强与成骨细胞祖细胞分化相关的关键成骨细胞特异性标志物表达的假设。将来自实验生物活性玻璃（6 P53-B）和商业生物玻璃（45 S5）的离子作为补充离子提取物（玻璃条件培养基（GCM））添加到成骨细胞祖细胞（MC 3 T3-E1.4）培养物中。离子提取物（Si：47.9 ± 10.4 ppm，Ca：69.8 ± 14.0（对于45 S5）; Si：33.4 ± 3.8 ppm，Ca：57.1 ± 2.8 ppm（对于6 P53-B））和对照浸提液（Si：< 0.1 ppm，Ca：49.0 ppm，在α-MEM中）补充（10%FBS，1%青霉素-链霉素），并添加到前成骨细胞（MC 3 T3-E1.4）培养物中。加入45 S5和6 P53-B GCM后，细胞增殖率在前三天内增加（为对照的150%）。对于分化培养物（GCM或对照培养基+抗坏血酸（50 mg L−1）），暴露于GCM 10天后，骨钙素蛋白表达增加至40×−70×对照，基因水平的表达水平相应增加（第3天，45 S5 GCM：14×对照;第5天，6 P53-B GCM：19×对照）。暴露于GCM 7天后，Runx 2水平约为对照的2倍。最后，在暴露1天后（Col 1 α1，45 S5 GCM：对照的3倍; 6 P53-B GCM：对照的4倍）和整个分化过程中（第5天，Col 1 α2，45 S5 GCM：对照的3.15倍; 6 P53-B GCM：对照的2.35倍），1型胶原表达增强。
This study tested the hypothesis that bioactive glass used for implant coatings enhance the expression of key osteoblast-specific markers associated with osteoblast progenitor differentiation. The ions from experimental bioactive glass (6P53-b) and commercial Bioglass (45S5) were added to osteoblast progenitor (MC3T3-E1.4) cultures as a supplemented ion extract (glass conditioned medium (GCM)). Ion extracts (Si: 47.9 ± 10.4 ppm, Ca: 69.8 ± 14.0 for 45S5; Si: 33.4 ± 3.8 ppm, Ca: 57.1 ± 2.8 ppm for 6P53-b) and control extract (Si: < 0.1 ppm, Ca: 49.0 ppm in α-MEM) were supplemented (10%FBS, 1% pen-strep) and added to pre-osteoblast (MC3T3-E1.4) cultures. Cell proliferation rate was enhanced (150% of control) within the first three days after adding 45S5 and 6P53-b GCM. For differentiating cultures (GCM or control medium + ascorbic acid (50 mg L−1)), osteocalcin protein expression increased to 40×−70× control after 10 days exposure to GCM with corresponding expression level increases at the gene level (day 3, 45S5 GCM: 14× control; day 5, 6P53-b GCM: 19× control). Runx2 levels were approximately 2× control after 7 days exposure to GCM. Finally, collagen type 1 expression was enhanced after 1 day of exposure (Col1α1, 45S5 GCM: 3x of control; 6P53-b GCM: 4× control) and on through the course of differentiation (day 5, Col1α2, 45S5 GCM: 3.15× control; 6P53-b GCM: 2.35× control)
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影响因子：
9.7
作者：
Foppiano, Silvia;Marshall, Sally J.;Tomsia, Antoni P.
通讯作者：
Tomsia, Antoni P.
DOI：
10.1002/jbm.10053
发表时间：
2001-12-05
期刊：
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH
影响因子：
--
作者：
Jones, JR;Sepulveda, P;Hench, LL
通讯作者：
Hench, LL
影响因子：
4.8
作者：
D'Alonzo, RC;Kowalski, AJ;Partridge, NC
通讯作者：
Partridge, NC
影响因子：
--
作者：
Bielby, RC;Christodoulou, IS;Polak, JM
通讯作者：
Polak, JM
影响因子：
56.9
作者：
HENCH, LL;WILSON, J
通讯作者：
WILSON, J