An automated controlled-release device for intravaginal hormone delivery.
An automated controlled-release device for intravaginal hormone delivery.
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DOI：
10.3168/jdsc.2020-18816
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发表时间：
2020-08
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影响因子：
--
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总结：我们开发并验证了一种用于牛生殖控制的电子控制的前列腺素给药装置，并证明了通过前列腺素F2α自动治疗诱导黄体退化的可行性。在农场使用这种自动化的输送系统可以简化畜群管理，减少动物的干扰，并改善排卵同步协议。我们开发并验证了一种全自动阴道内给药装置。我们的原型阴道内前列腺素释放装置在自动释放PGF 2？后成功诱导黄体消退。我们的自动化激素输送装置可能是目前激素管理方法的替代工具。我们的目标是开发和验证一种用于牛生殖控制的电子控制注射器。在阴道内（IVG）激素释放原型装置的开发和体外测试后，我们旨在证明通过PGF 2 α自动治疗诱导黄体退化的可行性。IVG设备包括外部3D打印塑料外壳、连接到输送泵和管道的流体储存器、可编程电路板和保持机构。对于体外试验，对4台泵进行编程，以释放不同的目标体积（0.1、0.2、0.5、1.0和2.0 mL），重复4次（n = 80）。构建了Bland-Altman图，以评估预期体积和输送体积之间的差异程度。观察结果在可接受的一致性范围内（1.96标准差）>95%的时间，表明总体一致性良好（平均差异=-0.005 mL）。为了评估IVG装置的体内性能，将至少有1个直径≥15 mm的黄体的泌乳荷斯坦奶牛随机分配至3种治疗之一：（1）IM-PGF（n = 6）：两次25 mg PGF 2 α肌内给药，间隔24 h;（2）DEV-PGF（n = 6）：IVG装置以10或12 h间隔自动释放4次25 mg剂量的PGF 2 α;和（3）DEV-CTL（n = 4）：插入空IVG装置（安慰剂对照）。在给药后0、12、24、36、48和72 h采集血样。数据采用重复测量的方差分析进行分析。所有器械（10/10）保持原位，直至48 h取出。从0到72小时的孕酮（P4）浓度受治疗，时间和它们的相互作用。P4浓度在时间0时无差异，但在24 - 72 h时不同：IM-PGF和DEV-PGF组奶牛的P4低于DEV-CTL组奶牛。相反，在实验期间，P4对于IM-PGF和DEV-PGF没有差异。我们的结论是，目前的IVG释放装置原型可以编程自动释放PGF 2 α，成功诱导泌乳奶牛黄体退化。
Summary: We developed and validated an electronically controlled hormone-delivery device for reproductive control of cattle and demonstrated the feasibility of inducing luteal regression by automated treatment with prostaglandin F2α. On-farm use of this automated delivery system might simplify herd management, reduce animal disruption, and enable improved ovulation synchronization protocols. We developed and validated a fully automated intravaginal hormone-delivery device. Our prototype intravaginal hormone-delivery device successfully induced luteal regression after automated release of PGF2?. Our automated hormone-delivery device may be an alternative tool to current methods for hormone administration. Our objective was to develop and validate an electronically controlled hormone-delivery device for reproductive control of cattle. After development and in vitro testing of a prototype device for intravaginal (IVG) hormone release, we aimed to demonstrate the feasibility of inducing luteal regression by automated treatment with PGF2α. The IVG device comprises an outer 3D-printed plastic housing, fluid reservoirs connected to delivery pumps and tubing, a programmable circuit board, and a retention mechanism. For in vitro testing, 4 pumps were programmed to release different target volumes (0.1, 0.2, 0.5, 1.0, and 2.0 mL) in 4 replicates (n = 80). A Bland-Altman plot was constructed to assess the magnitude of disagreement between expected and delivered volumes. Observations fell within acceptable limits of agreement (1.96 standard deviations) >95% of the time, indicating overall good agreement (mean difference = −0.005 mL). To assess in vivo performance of the IVG device, lactating Holstein cows with at least 1 corpus luteum ≥15 mm in diameter were randomly allocated to 1 of 3 treatments: (1) IM-PGF (n = 6): two 25-mg intramuscular doses of PGF2α 24 h apart; (2) DEV-PGF (n = 6): four 25-mg doses of PGF2α released automatically by the IVG device at 10- or 12-h intervals; and (3) DEV-CTL (n = 4): insertion of an empty IVG device (placebo control). Blood samples were collected at 0, 12, 24, 36, 48, and 72 h after treatment. Data were analyzed by ANOVA with repeated measures. All devices (10/10) remained in situ until removed at 48 h. Progesterone (P4) concentrations from 0 to 72 h were affected by treatment, time, and their interaction. Concentrations of P4 did not differ at time 0 but differed from 24 to 72 h: cows in IM-PGF and DEV-PGF had lesser P4 than cows in DEV-CTL. Conversely, P4 did not differ for IM-PGF and DEV-PGF during the experiment. We conclude that the current IVG hormone-releasing device prototype can be programmed to automatically release PGF2α for successful induction of luteal regression in lactating dairy cows.
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影响因子：
3.6
作者：
Beam, SW;Butler, WR
通讯作者：
Butler, WR
DOI：
10.1016/j.cvfa.2004.06.008
发表时间：
2004-11-01
影响因子：
4.1
作者：
Sheldon, M
通讯作者：
Sheldon, M
影响因子：
2.8
作者：
Ginther, O. J.;Shrestha, H. K.;Beg, M. A.
通讯作者：
Beg, M. A.
影响因子：
5.8
作者：
Cross, PS;Künnemeyer, R;Rathbone, MJ
通讯作者：
Rathbone, MJ
影响因子：
3.5
作者：
Wijma, R.;Stangaferro, M. L.;Giordano, J. O.
通讯作者：
Giordano, J. O.