Comparative Transcriptome Analysis of Pseudomonas putida KT2440 Revealed Its Response Mechanisms to Elevated Levels of Zinc Stress.
Comparative Transcriptome Analysis of Pseudomonas putida KT2440 Revealed Its Response Mechanisms to Elevated Levels of Zinc Stress.
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恶臭假单胞菌 KT2440 的比较转录组分析揭示了其对锌胁迫水平升高的反应机制
DOI：
10.3389/fmicb.2018.01669
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发表时间：
2018
影响因子：
5.2
通讯作者：
Liu P
中科院分区：
文献类型：
--
作者：
Peng J;Miao L;Chen X;Liu P
本研究通过 RNA 测序分析了恶臭假单胞菌 KT2440 对锌胁迫诱导浓度的全基因组转录反应，以彻底研究细菌细胞对锌毒性的反应。数据显示，不同水平的锌应激强烈影响以下类别基因的转录：金属转运基因、参与膜稳态的基因、氧化应激反应基因以及与基本细胞代谢相关的基因。在最低的锌剂量下，只有几个与金属转运和膜稳态相关的基因受到强烈影响。在中间锌剂量下，属于这两类的基因的转录变化非常明显。此外，中间锌胁迫产生高水平的氧化应激，影响恶臭假单胞菌的氨基酸代谢和呼吸链。在最高锌剂量下，最显着的特征是诱导负责 Fe-S 簇生物发生的基因。此外，观察到乙醛酸循环的上调。综上所述，细胞膜的适应、金属稳态和细胞内氧化还原状态的维持以及代谢的转录控制是应激反应的主要要素，有利于恶臭假单胞菌KT2440在锌污染环境中的生存。
The whole-genome transcriptional response of Pseudomonas putida KT2440 to stress-inducing concentrations of zinc was analyzed in this study by RNA sequencing to thoroughly investigate the bacterial cell response to zinc toxicity. The data revealed that different levels of zinc stress strongly affected the transcription of genes from the following categories: metal transport genes, genes involved in membrane homeostasis, oxidative-stress-responding genes, and genes associated with basic cellular metabolism. At the lowest zinc dose, only several genes associated with metal transport and membrane homeostasis were strongly influenced. At the intermediate zinc dose, transcriptional changes of genes belonging to these two categories were highly pronounced. In addition, the intermediate zinc stress produced high levels of oxidative stress, and influenced amino acid metabolism and respiratory chains of P. putida. At the highest zinc dose, the induction of genes responsible for Fe–S cluster biogenesis was the most remarkable feature. Moreover, upregulation of glyoxylate cycle was observed. In summary, the adaptation of the cell envelope, the maintenance of metal homeostasis and intracellular redox status, and the transcriptional control of metabolism are the main elements of stress response, which facilitates the survival of P. putida KT2440 in zinc-polluted environments.
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影响因子：
5.2
作者：
Barnett JP;Millard A;Ksibe AZ;Scanlan DJ;Schmid R;Blindauer CA
通讯作者：
Blindauer CA
影响因子：
6.4
作者：
Follonier S;Escapa IF;Fonseca PM;Henes B;Panke S;Zinn M;Prieto MA
通讯作者：
Prieto MA
影响因子：
2.8
作者：
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通讯作者：
Gadd, Geoffrey Michael
影响因子：
--
作者：
Barchinger, Sarah E;Ades, Sarah E
通讯作者：
Ades, Sarah E
影响因子：
13.8
作者：
Grabowicz M;Silhavy TJ
通讯作者：
Silhavy TJ