VAMP4 is required to maintain the ribbon structure of the Golgi apparatus.
VAMP4 is required to maintain the ribbon structure of the Golgi apparatus.
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DOI：
10.1007/s11010-013-1652-4
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发表时间：
2013-08
影响因子：
4.3
通讯作者：
Takuma, Taishin
中科院分区：
文献类型：
--
作者：
Shitara, Akiko;Shibui, Toru;Okayama, Miki;Arakawa, Toshiya;Mizoguchi, Itaru;Shakakura, Yasunori;Takuma, Taishin
高尔基体在脊椎动物细胞的核旁区域形成一个扭曲的带状网络。囊泡相关膜蛋白4(VAMP4)是一种仅在脊椎动物跨高尔基体网络(TGN)中表达的v-SNARE蛋白，在早期内体向TGN的逆行运输中发挥作用，但其在高尔基体中的确切功能尚不清楚。为了确定VAMP4是否在维持高尔基体结构中发挥功能作用，我们利用RNA干扰技术去除了VAMP4基因的表达。HeLa细胞中VAMP4的缺失导致高尔基体条带的断裂。这些碎片并不均匀地分布在细胞质中，但仍留在核旁区域。电子显微镜和免疫组织化学显示，在没有VAMP4的情况下，高尔基体堆积的长度缩短，但高尔基体堆积正常。在含有完整微管阵列的VAMP4耗竭细胞中，顺行转运没有受到损害。VAMP4、Synaxin 6、Synaxin 16和Vti1a的同源SNARs伙伴的枯竭也破坏了高尔基带结构。我们的研究结果表明，高尔基体结构的维持需要从早期的内体到TGN的正常逆行运输，这可能是由含有VAMP4的SNARE复合体的形成所介导的。
The Golgi apparatus forms a twisted ribbon-like network in the juxtanuclear region of vertebrate cells. Vesicle-associated membrane protein 4 (VAMP4), a v-SNARE protein expressed exclusively in the vertebrate trans-Golgi network (TGN), plays a role in retrograde trafficking from the early endosome to the TGN, although its precise function within the Golgi apparatus remains unclear. To determine whether VAMP4 plays a functional role in maintaining the structure of the Golgi apparatus, we depleted VAMP4 gene expression using RNA interference technology. Depletion of VAMP4 from HeLa cells led to fragmentation of the Golgi ribbon. These fragments were not uniformly distributed throughout the cytoplasm, but remained in the juxtanuclear area. Electron microscopy and immunohistochemistry showed that in the absence of VAMP4, the length of the Golgi stack was shortened, but Golgi stacking was normal. Anterograde trafficking was not impaired in VAMP4-depleted cells, which contained intact microtubule arrays. Depletion of the cognate SNARE partners of VAMP4, syntaxin 6, syntaxin 16, and Vti1a also disrupted the Golgi ribbon structure. Our findings suggested that the maintenance of Golgi ribbon structure requires normal retrograde trafficking from the early endosome to the TGN, which is likely to be mediated by the formation of VAMP4-containing SNARE complexes.
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影响因子：
7.8
作者：
GRIFFITHS, G;PFEIFFER, S;SIMONS, K;MATLIN, K
通讯作者：
MATLIN, K
影响因子：
3.3
作者：
Marra, Pierfrancesco;Salvatore, Lorena;De Matteis, Maria Antonietta
通讯作者：
De Matteis, Maria Antonietta
语法16和语法5是有效地逆行几种外源和内源性货物蛋白的逆行运输所必需的。
影响因子：
4
作者：
通讯作者：
--
影响因子：
6.6
作者：
Polishchuk, RS;Polishchuk, EV;Mironov, AA
通讯作者：
Mironov, AA
DOI：
10.1083/jcb.116.5.1071
发表时间：
1992-03
期刊：
The Journal of cell biology
影响因子：
--
作者：
Klausner RD;Donaldson JG;Lippincott-Schwartz J
通讯作者：
Lippincott-Schwartz J