Square prism micropillars on poly(methyl methacrylate) surfaces modulate the morphology and differentiation of human dental pulp mesenchymal stem cells.
Square prism micropillars on poly(methyl methacrylate) surfaces modulate the morphology and differentiation of human dental pulp mesenchymal stem cells.
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DOI：
10.1016/j.colsurfb.2019.02.037
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发表时间：
2019-06-01
期刊：
影响因子：
--
通讯作者：
Hasirci V
中科院分区：
文献类型：
--
作者：
Hasturk O;Ermis M;Demirci U;Hasirci N;Hasirci V
利用可溶性因子是体外诱导间充质干细胞（MSCs）向成骨细胞分化的最常用策略，但其在体内可能会增加潜在的副作用。基底表面的形貌影响细胞行为，这可能是一种有前途的指导干细胞分化的方法。据报道，微柱调节细胞和亚细胞的形状，它是特别有趣的，以调查这些变化在细胞形态是否可以调节基因表达和谱系承诺没有化学诱导。在聚甲基丙烯酸甲酯（PMMA）薄膜表面修饰了不同尺寸（4、8和16 μm）的方柱微柱，并通过氧等离子体处理改变了基底的表面润湿性。图案尺寸和亲水性均影响人牙髓间充质干细胞（DPSC）的附着、增殖，最重要的是影响其基因表达。减小柱宽和柱间距可促进细胞贴壁、细胞伸长和核变形，但降低早期增殖率。4或8 μm宽的支柱/间隙的表面在28天的培养中上调了早期骨标记基因的表达和矿化。暴露于氧等离子体增加润湿性，促进细胞附着和增殖，但延迟成骨。我们的研究结果表明，表面形貌和化学是控制基底上细胞行为的非常有用的工具，它们甚至可以帮助制造更好的植入物。最重要的发现是聚合物基底表面上的疏水微柱可以用于诱导MSC的成骨分化而无需任何分化补充剂。
Use of soluble factors is the most common strategy to induce osteogenic differentiation of mesenchymal stem cells (MSCs) in vitro, but it may raise potential side effects in vivo. The topographies of the substrate surfaces affect cell behavior, and this could be a promising approach to guide stem cell differentiation. Micropillars have been reported to modulate cellular and subcellular shape, and it is particularly interesting to investigate whether these changes in cell morphology can modulate gene expression and lineage commitment without chemical induction. In this study, poly(methyl methacrylate) (PMMA) films were decorated with square prism micropillars with different lateral dimensions (4, 8 and 16 μm), and the surface wettability of the substrates was altered by oxygen plasma treatment. Both pattern dimensions and hydrophilicity were found to affect the attachment, proliferation, and most importantly, gene expression of human dental pulp mesenchymal stem cells (DPSCs). Decreasing the pillar width and interpillar spacing enhanced cell attachment, cell elongation, and nuclear deformation of nuclei, but reduced early proliferation rate. Surfaces with 4 or 8 μm wide pillars/gaps upregulated the expression of early bone-marker genes and mineralization over 28 days of culture. Exposure to oxygen plasma increased wettability and promoted cell attachment and proliferation but delayed osteogenesis. Our findings showed that surface topography and chemistry are very useful tools in controlling cell behavior on substrates and they can even help create better implants. The most important finding is that hydrophobic micropillars on polymeric substrate surfaces can be exploited in inducing osteogenic differentiation of MSCs without any differentiation supplements.
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