Age-related differences in the respiratory microbiota of chickens.
Age-related differences in the respiratory microbiota of chickens.
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DOI：
10.1371/journal.pone.0188455
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Vervelde L
中科院分区：
文献类型：
--
作者：
Glendinning L;McLachlan G;Vervelde L
在下一代测序技术的时代，现在可以在没有传统培养技术固有的偏见的情况下对鸡呼吸道微生物群进行测序。然而，在这方面的研究很少。在这项研究中，我们使用16 S rRNA基因分析对来自三个不同年龄组的鸡的口腔、鼻腔和肺部微生物群样本进行了分析和比较。从2日龄（n = 5）、3周龄（n = 5）和30月龄（n = 6）的鸡中采集颊拭子和鼻拭子。还收集了支气管肺泡灌洗（BAL）样本以及仅含试剂的对照品。从这些样品中提取DNA，扩增16 S rRNA基因的V2-V3区并测序。在mothur中进行质量控制和OTU聚类。使用qPCR定量细菌DNA，扩增16 S rRNA基因的V3区。我们发现在三个不同的呼吸道部位采样的细菌数量和类型之间存在显着差异。我们还发现不同年龄组之间口腔、鼻腔和BAL液样本中细菌群落的组成、丰富度和多样性存在显著差异。我们鉴定了几种以前在基于培养的研究中从鸡呼吸道分离的细菌，包括乳酸杆菌和葡萄球菌。然而，我们还鉴定出以前从未从健康鸡的呼吸道中培养出的细菌。我们的结论是，我们的研究可以作为未来鸡呼吸道微生物群研究的基础。
In this era of next generation sequencing technologies it is now possible to characterise the chicken respiratory microbiota without the biases inherent to traditional culturing techniques. However, little research has been performed in this area. In this study we characterise and compare buccal, nasal and lung microbiota samples from chickens in three different age groups using 16S rRNA gene analysis. Buccal and nasal swabs were taken from birds aged 2 days (n = 5), 3 weeks (n = 5) and 30 months (n = 6). Bronchoalveolar lavage (BAL) samples were also collected alongside reagent only controls. DNA was extracted from these samples and the V2-V3 region of the 16S rRNA gene was amplified and sequenced. Quality control and OTU clustering were performed in mothur. Bacterial DNA was quantified using qPCR, amplifying the V3 region of the 16S rRNA gene. We found significant differences between the quantity and types of bacteria sampled at the three different respiratory sites. We also found significant differences in the composition, richness and diversity of the bacterial communities in buccal, nasal and BAL fluid samples between age groups. We identified several bacteria which had previously been isolated from the chicken respiratory tract in culture based studies, including lactobacilli and staphylococci. However, we also identified bacteria which have not previously been cultured from the respiratory tract of the healthy chicken. We conclude that our study can be used as a baseline that future chicken respiratory microbiota studies can build upon.
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影响因子：
4.8
作者：
Reiss-Mandel, Aylana;Regev-Yochay, Gili
通讯作者：
Regev-Yochay, Gili
影响因子：
4.4
作者：
Glendinning L;Wright S;Pollock J;Tennant P;Collie D;McLachlan G
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McLachlan G
影响因子：
18.2
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Dickson RP;Erb-Downward JR;Martinez FJ;Huffnagle GB
通讯作者：
Huffnagle GB
影响因子：
4.4
作者：
Glendinning L;Wright S;Tennant P;Gill AC;Collie D;McLachlan G
通讯作者：
McLachlan G
影响因子：
4.4
作者：
Schloss, Patrick D.;Westcott, Sarah L.;Weber, Carolyn F.
通讯作者：
Weber, Carolyn F.