Cochaperonin CPN20 negatively regulates abscisic acid signaling in Arabidopsis.

Cochaperonin CPN20 negatively regulates abscisic acid signaling in Arabidopsis.
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辅伴侣蛋白 CPN20 负向调节拟南芥中的脱落酸信号传导。

DOI:
10.1007/s11103-013-0082-8
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发表时间:
2013-10
影响因子:
5.1
通讯作者:
Zhang, Da-Peng
Zhang, Da-Peng
中科院分区:
生物学2区
文献类型:
--
作者:
Zhang, Xiao-Feng;Jiang, Tao;Wu, Zhen;Du, Shu-Yuan;Yu, Yong-Tao;Jiang, Shang-Chuan;Lu, Kai;Feng, Xiu-Jing;Wang, Xiao-Fang;Zhang, Da-Peng

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已有研究表明,镁-原卟啉IX螯合酶H亚基(CHLH/ABAR)正调控脱落酸(ABA)信号通路。本文研究了拟南芥CHLH/ABAR相互作用蛋白叶绿体共伴侣蛋白20 (CPN20)在ABA信号传导中的作用。结果表明,CPN20基因的下调表达增加,而CPN20基因的过表达减少,在ABA诱导的种子萌发抑制、萌发后生长停滞、促进气孔关闭和抑制气孔开放等主要ABA反应中,对ABA的敏感性增加。遗传学证据支持CPN20在CHLH/ABAR的下游或同一节点上起作用,但在WRKY40转录因子的上游。其他CPN20相互作用伙伴CPN10和CPN60不参与ABA信号传导。我们的研究结果表明,CPN20在ABAR-WRKY40偶联ABA信号传导中负向作用,独立于其共伴侣蛋白的作用,并为共伴侣蛋白在调节植物对环境信号的反应中的作用提供了新的见解。本文的在线版本(doi:10.1007/s11103-013-0082-8)包含补充资料,仅供授权用户使用。
Previous study showed that the magnesium-protoporphyrin IX chelatase H subunit (CHLH/ABAR) positively regulates abscisic acid (ABA) signaling. Here, we investigated the functions of a CHLH/ABAR interaction protein, the chloroplast co-chaperonin 20 (CPN20) in ABA signaling in Arabidopsis thaliana. We showed that down-expression of the CPN20 gene increases, but overexpression of the CPN20 gene reduces, ABA sensitivity in the major ABA responses including ABA-induced seed germination inhibition, postgermination growth arrest, promotion of stomatal closure and inhibition of stomatal opening. Genetic evidence supports that CPN20 functions downstream or at the same node of CHLH/ABAR, but upstream of the WRKY40 transcription factor. The other CPN20 interaction partners CPN10 and CPN60 are not involved in ABA signaling. Our findings show that CPN20 functions negatively in the ABAR-WRKY40 coupled ABA signaling independently of its co-chaperonin role, and provide a new insight into the role of co-chaperones in the regulation of plant responses to environmental cues. The online version of this article (doi:10.1007/s11103-013-0082-8) contains supplementary material, which is available to authorized users.
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