The non-invasive measurement of faecal immunoglobulin in African equids
The non-invasive measurement of faecal immunoglobulin in African equids
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非洲马科动物粪便免疫球蛋白的无创测量
DOI：
10.1016/j.ijppaw.2020.05.005
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Rubenstein, Daniel I.
中科院分区：
文献类型：
--
作者：
Tombak, Kaia J.;Budischak, Sarah A.;Hauck, Stephanie;Martinez, Lindsay A.;Rubenstein, Daniel I.
生态免疫学研究受到缺乏野生环境基础研究和几乎没有非侵入性工具来测量野生动物内部状态的阻碍。最近开发的酶联免疫吸附试验，用于测量免疫球蛋白的粪便样品Soay绵羊促使我们优化这样的测定来测量免疫球蛋白A（伊加：抗体与寄生线虫繁殖力）的粪便样品从马。我们测量了国内驴，野生平原斑马，野生Grevy的斑马共享相同的景观在肯尼亚中部超过两个领域的季节总伊加。尝试测量抗线虫伊加更具体地说，使用从排泄线虫的混合物的均质化提取物，未能清除背景。然而，我们发现，总伊加呈正相关，与圆线虫粪便卵计数（粪便中的粪便中此外，这种关系出现在高身体状况的驴，但没有在低身体状况的驴。在重复采样的驴中，伊加和FEC测量值之间的1-4天的时间滞后并没有产生相关性，这表明伊加和FEC大致相互跟踪，在潮湿的季节没有太多的延迟。这种直接的IgA-FEC的关系没有出现斑马在潮湿或干燥的领域的季节，可能是由于较高的个体间变异的身体状况之间的自由漫游斑马比驴。然而，Grevy的斑马比平原斑马或驴的伊加水平更高，这可能与它们在种群水平上较低的FEC有关。我们的研究结果表明，马可能会安装伊加响应线虫卵生产时，主机是在良好的条件下，马种可能不同的粘膜伊加的基线水平。
Eco-immunological research is encumbered by a lack of basic research in a wild context and by the availability of few non-invasive tools to measure the internal state of wild animals. The recent development of an enzyme-linked immunosorbent assay for measuring immunoglobulins in faecal samples from Soay sheep prompted us to optimize such an assay to measure immunoglobulin A (IgA: an antibody associated with parasitic nematode fecundity) in faecal samples from equids. We measured total IgA in domestic donkeys, wild plains zebras, and wild Grevy's zebras sharing the same landscape in central Kenya over two field seasons. Attempts to measure anti-nematode IgA more specifically, using a homogenized extract from a mixture of excreted nematodes, failed to clear background. However, we found that total IgA positively correlated with strongyle nematode faecal egg counts (FECs) in donkeys sampled during the wetter field season - a time when the donkeys were in good condition. Further, this relationship appeared among donkeys with high body condition but not among those with low body condition. Time lags of 1–4 days introduced between IgA and FEC measurements in repeatedly sampled donkeys did not yield correlations, suggesting that IgA and FEC roughly tracked one another without much delay in the wet field season. Such a direct IgA-FEC relationship did not appear for zebras in either the wet or dry field season, possibly due to higher interindividual variation in body condition among the free-roaming zebras than in the donkeys. However, Grevy's zebras had higher overall levels of IgA than either plains zebras or donkeys, potentially associated with their reportedly lower FECs at the population level. Our results suggest that equids may mount an IgA response to nematode egg production when the host is in good condition and that equid species may differ in baseline levels of mucosal IgA.
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影响因子：
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作者：
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