Low-Temperature Adaptation Targets Genome Packing Reactions in an Icosahedral Single-Stranded DNA Virus
Low-Temperature Adaptation Targets Genome Packing Reactions in an Icosahedral Single-Stranded DNA Virus
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低温适应以二十面体单链 DNA 病毒中的基因组包装反应为目标
DOI：
10.1128/jvi.01970-21
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发表时间：
2022
影响因子：
5.4
通讯作者：
Fane, Bentley A.
中科院分区：
文献类型：
--
作者：
Ogunbunmi, Elizabeth T.;Love, Samuel D.;Rhodes, Katherine A.;Morales, Adriana;Wilch, Margaret H.;Jonas, Jeremy;Fane, Bentley A.
X174、G4和α3代表一个微病毒科的三个姐妹属。类似α3的基因组比它们的姊妹属基因组大得多，但它们被包装成内部体积相似的衣壳。它们还包含多个A*基因，这些基因嵌套在更大的A基因阅读框架内。虽然在大多数情况下不是必需的，但A*蛋白调节包装反应的保真度。更大的基因组和多个A*基因可能表明，基因组包装对类似α3的病毒来说更有问题，特别是在温度较低的情况下，那里的dna持续长度会更长。与其他属的成员在20℃下可靠地形成斑块不同，α3类噬菌体在28℃以下自然对冷敏感。为了确定独特的α3类基因组特征与冷敏感表型之间是否存在联系，我们在低温下对α3组装途径进行了表征。虽然没有检测到病毒粒子，但观察到了与非路径包装复合体一致的颗粒。在一种互补的进化方法中，α3被实验进化为在逐渐较低的温度下生长。冷适应的两个主要反应是基因组减少和A*基因表达增加。重要的是，酶、转录因子和病毒受体的产生直接影响病毒可以栖息的生态位。一些原核生物宿主可以在差异很大的环境中茁壮成长；因此，还应该考虑温度等物理参数。这些变量可能会直接改变宿主的生理，从而阻止病毒复制。或者，它们可以以非宿主的方式负向抑制感染过程。小病毒属(Microviridaegenera)的三个姊妹种(典型的小病毒属(Microviridaegenera)有三个成员，分别是典型的物种øx174、G4和α3)感染同一个宿主，但类α3病毒具有天然的冷敏感性，这可以有效地将它们排除在低温环境(<；28°C)之外。排斥性似乎与宿主细胞的生理无关。相反，它可能在很大程度上归因于低温包装缺陷。这里提出的结果表明，物理参数，如温度，可以直接影响病毒的多样性和以非宿主方式确定生态位。
øX174, G4, and α3 represent the three sister genera of aMicroviridaesubfamily. α3-like genomes are considerably larger than their sister genera genomes, yet they are packaged into capsids of similar internal volumes. They also contain multiple A* genes, which are nested within the larger A gene reading frame. Although unessential under most conditions, A* proteins mediate the fidelity of packaging reactions. Larger genomes and multiple A* genes may indicate that genome packaging is more problematic for α3-like viruses, especially at lower temperatures, where DNA persistence lengths would be longer. Unlike members of the other genera, which reliably form plaques at 20°C, α3-like phages are naturally cold sensitive below 28°C. To determine whether there was a connection between the uniquely α3-like genome characteristics and the cold-sensitive phenotype, the α3 assembly pathway was characterized at low temperature. Although virions were not detected, particles consistent with off-pathway packaging complexes were observed. In a complementary evolutionary approach, α3 was experimentally evolved to grow at progressively lower temperatures. The two major responses to cold adaptation were genome reduction and elevated A* gene expression.IMPORTANCEThe production of enzymes, transcription factors, and viral receptors directly influences the niches viruses can inhabit. Some prokaryotic hosts can thrive in widely differing environments; thus, physical parameters, such as temperature, should also be considered. These variables may directly alter host physiology, preventing viral replication. Alternatively, they could negatively inhibit infection processes in a host-independent manner. The members of three sisterMicroviridaegenera (canonical species øX174, G4 and α3) infect the same host, but α3-like viruses are naturally cold sensitive, which could effectively exclude them from low-temperature environments (<28°C). Exclusion appeared to be independent of host cell physiology. Instead, it could be largely attributed to low-temperature packaging defects. The results presented here demonstrate how physical parameters, such as temperature, can directly influence viral diversification and niche determination in a host-independent manner.
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大规模染色体折叠与基因组 DNA 序列：离散双傅里叶变换技术。
影响因子：
2
作者：
V. R. Chechetkin;V. Lobzin
通讯作者：
V. Lobzin
DOI：
10.1080/07391102.2018.1479660
发表时间：
2019-06-13
影响因子：
4.4
作者：
Chechetkin，V. R.;Lobzin，V. V.
通讯作者：
Lobzin，V. V.
DOI：
10.1073/pnas.73.10.3519
发表时间：
1976-01-01
影响因子：
11.1
作者：
HAYASHI, M;FUJIMURA, FK;HAYASHI, M
通讯作者：
HAYASHI, M
影响因子：
3.7
作者：
Mankertz, A;Hillenbrand, B
通讯作者：
Hillenbrand, B
φX174 基因 A 的两种蛋白质
DOI：
--
发表时间：
1973
期刊：
影响因子：
--
作者：
E. Linney;M. Hayashi
通讯作者：
M. Hayashi