Conservation of streptococcal CRISPRs on human skin and saliva.
Conservation of streptococcal CRISPRs on human skin and saliva.
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DOI：
10.1186/1471-2180-14-146
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发表时间：
2014-06-06
期刊：
影响因子：
4.2
通讯作者：
Pride DT
中科院分区：
文献类型：
--
作者：
Robles-Sikisaka R;Naidu M;Ly M;Salzman J;Abeles SR;Boehm TK;Pride DT
细菌利用成簇的规则间隔短回文重复序列(CRISPR)来抵抗与病毒的接触。人体表面有许多携带CRISPR的细菌，它们的内容可以为他们可能遇到的病毒的类型和特征提供线索。我们研究了链球菌在人类皮肤和唾液中的CRISPR含量在8周内的保存情况，以确定CRISPR间隔区是否存在相似性，以及CRISPR间隔区是否是每个生物地理位置的稳定成分。大多数鉴定的CRISPR序列是独特的，但来自每个受试者皮肤和唾液的一小部分间隔区与来自先前测序的嗜热链球菌和其他链球菌的间隔区相匹配。在整个8周的研究期间，所有受试者都有相当大比例的CRISPR间隔区是保守的，早上采样的唾液CRISPR间隔区显示出明显高于一天中任何其他时间的保守性水平。我们还发现每个受试者的皮肤和唾液的间隔区序列有很大的相似之处。许多皮肤衍生的间隔区与唾液病毒匹配，这表明皮肤上的细菌可能会遇到与口腔中发现的病毒序列相似的病毒。尽管皮肤和唾液间隔序列有相似之处，但根据受试者和身体部位的不同，存在的差异是不同的。在研究的一段时间内，人类受试者唾液和皮肤中CRISPR间隔区的保守表明，携带它们的细菌相对保守。
Clustered Regularly Interspaced Short Palindromic Repeats (CRISPRs) are utilized by bacteria to resist encounters with their viruses. Human body surfaces have numerous bacteria that harbor CRISPRs, and their content can provide clues as to the types and features of viruses they may have encountered. We investigated the conservation of CRISPR content from streptococci on skin and saliva of human subjects over 8-weeks to determine whether similarities existed in the CRISPR spacer profiles and whether CRISPR spacers were a stable component of each biogeographic site. Most of the CRISPR sequences identified were unique, but a small proportion of spacers from the skin and saliva of each subject matched spacers derived from previously sequenced loci of S. thermophilus and other streptococci. There were significant proportions of CRISPR spacers conserved over the entire 8-week study period for all subjects, and salivary CRISPR spacers sampled in the mornings showed significantly higher levels of conservation than any other time of day. We also found substantial similarities in the spacer repertoires of the skin and saliva of each subject. Many skin-derived spacers matched salivary viruses, supporting that bacteria of the skin may encounter viruses with similar sequences to those found in the mouth. Despite the similarities between skin and salivary spacer repertoires, the variation present was distinct based on each subject and body site. The conservation of CRISPR spacers in the saliva and the skin of human subjects over the time period studied suggests a relative conservation of the bacteria harboring them.
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DOI：
10.1073/pnas.1205624109
发表时间：
2012-05-22
影响因子：
11.1
作者：
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DOI：
10.1073/pnas.0807920105
发表时间：
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影响因子：
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影响因子：
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影响因子：
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作者：
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