Genomic Characterization Provides an Insight into the Pathogenicity of the Poplar Canker Bacterium Lonsdalea populi.
Genomic Characterization Provides an Insight into the Pathogenicity of the Poplar Canker Bacterium Lonsdalea populi.
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基因组表征有助于深入了解杨树溃疡病菌 Lonsdalea populi 的致病性
作者：
Chen X;Li R;Wang Y;Li A
摘要一种由革兰氏阴性菌（Lonsdalea populi）引起的新发杨树溃疡病导致中国和欧洲杂交杨树（Populus × euramericana）的高死亡率。白杨L. populi致病性和树皮适应性的分子基础已成为近年来研究的热点。本研究揭示了populi的全基因组序列，并确定了假定的毒力因子。重新构建了一个高质量的大众L.基因组序列，基因组大小为3,859,707 bp，包含约3434个基因和107个rna（75个tRNA， 22个rRNA和10个ncRNA）。L. populi基因组包含380个毒力相关基因，主要编码粘附、胞外酶、分泌系统和双组分转导系统。基因组有110个碳水化合物活性酶（CAZy）编码基因和推测的分泌蛋白。耐药数据库注释显示，L. populi对青霉素、氟喹诺酮和卡苏霉素耐药。比较基因组学分析发现，白杨与不列颠白杨基因组同源性最高，包含1905个特异性基因、1769个非必需基因和1381个保守基因，具有较高的进化多样性和基因组可塑性。泛基因组分析表明，N-5-1基因组为开放基因组。这些研究结果为进一步了解白杨L. populi和杨树-细菌互作的致病性和生物学的分子基础提供了重要的资源。
An emerging poplar canker caused by the gram-negative bacterium, Lonsdalea populi, has led to high mortality of hybrid poplars Populus × euramericana in China and Europe. The molecular bases of pathogenicity and bark adaptation of L. populi have become a focus of recent research. This study revealed the whole genome sequence and identified putative virulence factors of L. populi. A high-quality L. populi genome sequence was assembled de novo, with a genome size of 3,859,707 bp, containing approximately 3434 genes and 107 RNAs (75 tRNA, 22 rRNA, and 10 ncRNA). The L. populi genome contained 380 virulence-associated genes, mainly encoding for adhesion, extracellular enzymes, secretory systems, and two-component transduction systems. The genome had 110 carbohydrate-active enzyme (CAZy)-coding genes and putative secreted proteins. The antibiotic-resistance database annotation listed that L. populi was resistant to penicillin, fluoroquinolone, and kasugamycin. Analysis of comparative genomics found that L. populi exhibited the highest homology with the L. britannica genome and L. populi encompassed 1905 specific genes, 1769 dispensable genes, and 1381 conserved genes, suggesting high evolutionary diversity and genomic plasticity. Moreover, the pan genome analysis revealed that the N-5-1 genome is an open genome. These findings provide important resources for understanding the molecular basis of the pathogenicity and biology of L. populi and the poplar-bacterium interaction.
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