Listeria cossartiae sp. nov., Listeria farberi sp. nov., Listeria immobilis sp. nov., Listeria portnoyi sp. nov. and Listeria rustica sp. nov., isolated from agricultural water and natural environments.
Listeria cossartiae sp. nov., Listeria farberi sp. nov., Listeria immobilis sp. nov., Listeria portnoyi sp. nov. and Listeria rustica sp. nov., isolated from agricultural water and natural environments.
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DOI：
10.1099/ijsem.0.004795
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发表时间：
2021-05
影响因子：
2.8
通讯作者：
Wiedmann M
中科院分区：
文献类型：
--
作者：
Carlin CR;Liao J;Weller D;Guo X;Orsi R;Wiedmann M
从美国本土不同地点的土壤样本和纽约的农业水样中获得了总共 27 种无法分类到物种水平的李斯特菌分离株。基于全基因组序列的平均核苷酸同一性比对 (ANIb) 显示，27 个分离株形成 5 个不同的簇；对于每个簇，所有基因组草案显示彼此之间以及任何当前描述的李斯特菌物种的 ANI 值 <95 % 相似性，表明每个簇代表一个新物种。在这五个新物种中，三个属于李斯特氏菌属严格分支，两个属于李斯特菌属。一种新的严格意义上的物种，命名为 L. cossartiae sp。 11月，包含两个平均ANI相似度为94.9%的亚簇，被指定为亚种。所提议的三个新的严格意义上的物种（包括两个亚种）是李斯特菌farberi sp。十一月（典型菌株 FSL L7-0091T=CCUG 74668T=LMG 31917T；对无害李斯特氏菌最大 ANI 91.9 %）、固定李斯特菌 (Listeria immobilis sp.)。十一月（模式菌株 FSL L7-1519T=CCUG 74666T=LMG 31920T；L. ivanovii subsp. londoniensis 的最大 ANI 为 87.4 %）和 Listeria cossartiae sp。十一月[亚种。 cossartiae（典型菌株 FSL L7-1447T=CCUG 74667T=LMG 31919T；对 L. marthii 的最大 ANI 93.4 %）和亚种。 cayugensis（典型菌株 FSL L7-0993T=CCUG 74670T=LMG 31918T；对 L. marthii 的最大 ANI 为 94.7 %）。拟议的两个新的意义物种是李斯特菌 portnoyi sp。十一月（模式菌株 FSL L7-1582T=CCUG 74671T=LMG 31921T；L. cornellensis 的最大 ANI 值为 88.9 %，L. newyorkensis 的最大 ANI 值为 89.2 %）和 Listeria rustica sp。十一月（典型菌株 FSL W9-0585T=CCUG 74665T=LMG 31922T；L. cornellensis 的最大 ANI 值为 88.7 %，L. newyorkensis 的最大 ANI 值为 88.9 %）。 L. immobilis 是迄今为止分离出来的第一个非运动的严格意义上的物种。所有五个新物种均不具有溶血性，且磷脂酰肌醇特异性磷脂酶 C 活性呈阴性；基因组草图缺乏李斯特菌致病性岛 1 (LIPI-1) 中发现的毒力基因以及内素基因 inlA 和 inlB，表明它们不具有致病性。
A total of 27 Listeria isolates that could not be classified to the species level were obtained from soil samples from different locations in the contiguous United States and an agricultural water sample from New York. Whole-genome sequence-based average nucleotide identity blast (ANIb) showed that the 27 isolates form five distinct clusters; for each cluster, all draft genomes showed ANI values of <95 % similarity to each other and any currently described Listeria species, indicating that each cluster represents a novel species. Of the five novel species, three cluster with the Listeria sensu stricto clade and two cluster with sensu lato. One of the novel sensu stricto species, designated L. cossartiae sp. nov., contains two subclusters with an average ANI similarity of 94.9%, which were designated as subspecies. The proposed three novel sensu stricto species (including two subspecies) are Listeria farberi sp. nov. (type strain FSL L7-0091T=CCUG 74668T=LMG 31917T; maximum ANI 91.9 % to L. innocua ), Listeria immobilis sp. nov. (type strain FSL L7-1519T=CCUG 74666T=LMG 31920T; maximum ANI 87.4 % to L. ivanovii subsp. londoniensis ) and Listeria cossartiae sp. nov. [subsp. cossartiae (type strain FSL L7-1447T=CCUG 74667T=LMG 31919T; maximum ANI 93.4 % to L. marthii ) and subsp. cayugensis (type strain FSL L7-0993T=CCUG 74670T=LMG 31918T; maximum ANI 94.7 % to L. marthii ). The two proposed novel sensu lato species are Listeria portnoyi sp. nov. (type strain FSL L7-1582T=CCUG 74671T=LMG 31921T; maximum ANI value of 88.9 % to L. cornellensis and 89.2 % to L. newyorkensis ) and Listeria rustica sp. nov. (type strain FSL W9-0585T=CCUG 74665T=LMG 31922T; maximum ANI value of 88.7 % to L. cornellensis and 88.9 % to L . newyorkensis ). L. immobilis is the first sensu stricto species isolated to date that is non-motile. All five of the novel species are non-haemolytic and negative for phosphatidylinositol-specific phospholipase C activity; the draft genomes lack the virulence genes found in Listeria pathogenicity island 1 (LIPI-1), and the internalin genes inlA and inlB, indicating that they are non-pathogenic.
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DOI：
10.1099/ijs.0.048587-0
发表时间：
2013-09-01
影响因子：
2.8
作者：
den Bakker, Henk C.;Manuel, Clyde S.;Nightingale, Kendra K.
通讯作者：
Nightingale, Kendra K.
DOI：
10.1099/ijs.0.014118-0
发表时间：
2010-06-01
影响因子：
2.8
作者：
Graves, Lewis M.;Helsel, Leta O.;Sauders, Brian D.
通讯作者：
Sauders, Brian D.
影响因子：
4.4
作者：
CZAJKA, J;BSAT, N;BATT, CA
通讯作者：
BATT, CA
影响因子：
1.7
作者：
Bankevich, Anton;Nurk, Sergey;Pevzner, Pavel A.
通讯作者：
Pevzner, Pavel A.
DOI：
10.1099/ijs.0.052720-0
发表时间：
2014-06-01
影响因子：
2.8
作者：
den Bakker, Henk C.;Warchocki, Steven;Wiedmann, Martin
通讯作者：
Wiedmann, Martin