Over-expression of eukaryotic translation initiation factor 4 gamma 1 correlates with tumor progression and poor prognosis in nasopharyngeal carcinoma.
Over-expression of eukaryotic translation initiation factor 4 gamma 1 correlates with tumor progression and poor prognosis in nasopharyngeal carcinoma.
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真核翻译起始因子4γ1的过度表达与鼻咽癌的肿瘤进展和不良预后相关
DOI：
10.1186/1476-4598-9-78
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发表时间：
2010-04-16
期刊：
影响因子：
37.3
通讯作者：
Fang W
中科院分区：
文献类型：
--
作者：
Tu L;Liu Z;He X;He Y;Yang H;Jiang Q;Xie S;Xiao G;Li X;Yao K;Fang W
背景本研究的目的是分析真核翻译起始因子4γ1（EIF4G1）在鼻咽癌（NPC）中的表达及其与临床病理特征（包括患者生存时间）的相关性。方法采用实时荧光定量PCR（real-time PCR）检测EIF4G1在正常鼻咽组织、永生化鼻咽上皮细胞系NP69、鼻咽癌组织和细胞中的表达。使用免疫组织化学检查鼻咽癌组织中EIF4G1蛋白的表达。使用Kaplan-Meier方法进行生存分析。探讨EIF4G1对细胞侵袭和肿瘤发生的影响。结果鼻咽癌组织和细胞系中EIF4G1 mRNA的表达水平显着高于正常鼻咽组织和NP69细胞（P<0.001）。免疫组化分析显示，鼻咽癌组织中EIF4G1蛋白的表达高于鼻咽组织（P<0.001）。此外，肿瘤中EIF4G1蛋白水平与肿瘤T分型（P=0.039）、淋巴结受累情况（N分型，P=0.008）以及鼻咽癌患者的临床分期（P=0.003）呈正相关。 EIF4G1表达较高的患者总生存时间较短（P=0.019）。多因素分析显示EIF4G1表达是鼻咽癌患者总生存期的独立预后指标。使用shRNA敲低EIF4G1的表达不仅显着抑制细胞周期进展、增殖、迁移、侵袭和集落形成，而且还显着抑制体内异种移植肿瘤的生长。结论我们的数据表明EIF4G1可以作为鼻咽癌患者预后的生物标志物。
BackgroundThe aim of the present study was to analyze the expression of eukaryotic translation initiation factor 4 gamma 1 (EIF4G1) in nasopharyngeal carcinoma (NPC) and its correlation with clinicopathologic features, including patients' survival time.MethodsUsing real-time PCR, we detected the expression ofEIF4G1in normal nasopharyngeal tissues, immortalized nasopharyngeal epithelial cell lines NP69, NPC tissues and cell lines.EIF4G1protein expression in NPC tissues was examined using immunohistochemistry. Survival analysis was performed using Kaplan-Meier method. The effect ofEIF4G1on cell invasion and tumorigenesis were investigated.ResultsThe expression levels ofEIF4G1mRNA were significantly greater in NPC tissues and cell lines than those in the normal nasopharyngeal tissues and NP69 cells (P< 0.001). Immunohistochemical analysis revealed that the expression ofEIF4G1protein was higher in NPC tissues than that in the nasopharyngeal tissues (P< 0.001). In addition, the levels ofEIF4G1protein in tumors were positively correlated with tumor T classification (P= 0.039), lymph node involvement (N classification,P= 0.008), and the clinical stages (P= 0.003) of NPC patients. Patients with higherEIF4G1 expression had shorter overall survival time (P= 0.019). Multivariate analysis showed thatEIF4G1expression was an independent prognostic indicator for the overall survival of NPC patients. Using shRNA to knock down the expression ofEIF4G1not only markedly inhibited cell cycle progression, proliferation, migration, invasion, and colony formation, but also dramatically suppressedin vivoxenograft tumor growth.ConclusionOur data suggest thatEIF4G1can serve as a biomarker for the prognosis of NPC patients.
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DOI：
10.1002/hed.20833
发表时间：
2008-07
影响因子：
2.9
作者：
Chou, Josephine;Lin, Yu-Ching;Kim, Jae;You, Liang;Xu, Zhidong;He, Biao;Jablons, David M.
通讯作者：
Jablons, David M.
影响因子：
5.3
作者：
Yang, HS;Jansen, AP;Colburn, NH
通讯作者：
Colburn, NH
SATB2表达下调与结直肠癌转移和不良预后相关
影响因子：
7.3
作者：
Wang, Shuang;Zhou, Jun;Li, Jian-Ming
通讯作者：
Li, Jian-Ming
影响因子：
2.4
作者：
Özyar, E;Ayhan, A;Atahan, IL
通讯作者：
Atahan, IL
影响因子：
6.4
作者：
Bauer, C;Brass, N;Meese, E
通讯作者：
Meese, E