Identification of ceftazidime interaction with bacteria in wastewater treatment by Raman spectroscopic mapping.
Identification of ceftazidime interaction with bacteria in wastewater treatment by Raman spectroscopic mapping.
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通过拉曼光谱图鉴定废水处理中头孢他啶与细菌的相互作用
DOI：
10.1039/c9ra06006e
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发表时间：
2019-10-10
期刊：
影响因子：
3.9
通讯作者：
Guo, Jin-Song
中科院分区：
文献类型：
--
作者：
Peng, Meng-Wen;Wei, Xiang-Yang;Yu, Qiang;Yan, Peng;Chen, You-Peng;Guo, Jin-Song
拉曼光谱产生指纹光谱，并且在医学和生物科学中非常重要，因为它是非破坏性的、非侵入性的，并且在水性环境中可用。本研究利用拉曼光谱和拉曼图谱研究了头孢他啶胁迫下筛选菌的动态生化过程。采用主成分分析法分析了抗生素胁迫前后细菌的拉曼光谱差异，得到了细菌的特征峰。结果表明，头孢他啶胁迫下，细菌的氨基酸首先发生变化，脂类含量降低。此外，在拉曼图谱中，当细菌受到抗生素胁迫时，1002 cm−1处的峰（苯丙氨酸）增加，而1172 cm−1处的峰（脂质）减弱。这表明，当细菌被抗生素刺激时，细胞内脂质减少，特定氨基酸含量增加。细胞内脂质的减少可能提示膜通透性的改变。特定氨基酸的增加表明细菌通过调节相关酶的活性来抵抗抗生素的外部刺激。本研究利用拉曼光谱技术探讨了细菌与抗生素作用的过程，为今后进一步研究微生物生化过程的动力学奠定了基础。拉曼光谱产生指纹光谱，并且在医学和生物科学中非常重要，因为它是非破坏性的、非侵入性的，并且在水性环境中可用。
Raman spectroscopy yields a fingerprint spectrum and is of great importance in medical and biological sciences as it is non-destructive, non-invasive, and available in the aqueous environment. In this study, Raman spectroscopy and Raman mapping were used to explore the dynamic biochemical processes in screened bacteria under ceftazidime stress. The Raman spectral difference between bacteria with and without antibiotic stress was analyzed by principal component analysis and characteristic peaks were obtained. The results showed that amino acids changed first and lipids were reduced when bacteria were exposed to ceftazidime stress. Furthermore, in Raman mapping, when bacteria were subjected to antibiotic stress, the peak at 1002 cm−1 (phenylalanine) increased, while the peak at 1172 cm−1 (lipids) weakened. This indicates that when bacteria were stimulated by antibiotics, the intracellular lipids decreased and the content of specific amino acids increased. The reduction of intracellular lipids may suggest a change of membrane permeability. The increase of specific amino acids suggests that bacteria resist external stimuli of antibiotics by regulating the activities of related enzymes. This study explored the processes of the action between bacteria and antibiotics by Raman spectroscopy, and provides a foundation for the further study of the dynamics of microbial biochemical processes in the future. Raman spectroscopy yields a fingerprint spectrum and is of great importance in medical and biological sciences as it is non-destructive, non-invasive, and available in the aqueous environment.
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影响因子：
8.8
作者：
Mueller, Elisabeth;Schuessler, Walter;Lemmer, Hilde
通讯作者：
Lemmer, Hilde
影响因子：
2.9
作者：
Neugebauer, U.;Schmid, U.;Popp, J.
通讯作者：
Popp, J.
DOI：
10.1111/nyas.12847
发表时间：
2015-01-01
期刊：
ANTIMICROBIAL THERAPEUTICS REVIEWS
影响因子：
--
作者：
Carey, Paul R.;Heidari-Torkabadi, Hossein
通讯作者：
Heidari-Torkabadi, Hossein
影响因子：
3.5
作者：
Lau, Andy K. S.;Wong, Terence T. W.;Tsia, Kevin K.
通讯作者：
Tsia, Kevin K.
高分辨率的活细胞拉曼成像，使用靶向SERS敏感的金纳米颗粒具有高度窄的纳米纳图。
影响因子：
10.8
作者：
Kang JW;So PT;Dasari RR;Lim DK
通讯作者：
Lim DK