High resolution live cell Raman imaging using subcellular organelle-targeting SERS-sensitive gold nanoparticles with highly narrow intra-nanogap.
High resolution live cell Raman imaging using subcellular organelle-targeting SERS-sensitive gold nanoparticles with highly narrow intra-nanogap.
复制标题
高分辨率的活细胞拉曼成像，使用靶向SERS敏感的金纳米颗粒具有高度窄的纳米纳图。
DOI：
10.1021/nl504444w
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发表时间：
2015-03-11
期刊：
影响因子：
10.8
通讯作者：
Lim DK
中科院分区：
文献类型：
--
作者：
Kang JW;So PT;Dasari RR;Lim DK
我们报告了一种方法，以实现高速和高分辨率的活细胞拉曼图像使用小球形金纳米粒子与高度狭窄的内纳米间隙结构响应近红外激发（785 nm）和高速共焦拉曼显微镜。将三种不同的拉曼活性分子置于纳米间隙中，即使在短暂曝光时间（10 ms）和低入射激光功率（200 μW）下，溶液中也显示出强而均匀的拉曼强度，这导致在30 s内获得高分辨率的单细胞图像，而不会引起明显的细胞损伤。高分辨率的拉曼图像显示了金纳米颗粒在其靶向部位如细胞质、线粒体或细胞核的分布。基于高速拉曼的活细胞成像使我们能够监测在向细胞中添加高毒性KCN溶液诱导的细胞死亡期间快速变化的细胞形态。这些结果有力地表明，使用SERS活性纳米颗粒可以大大提高基于拉曼的细胞图像的当前时间分辨率和图像质量，足以获得详细的细胞动力学和/或细胞对潜在药物分子的响应。
We report a method to achieve high speed and high resolution live cell Raman images using small spherical gold nanoparticles with highly narrow intra-nanogap structures responding to NIR excitation (785 nm) and high-speed confocal Raman microscopy. The three different Raman-active molecules placed in the narrow intra-nanogap showed a strong and uniform Raman intensity in solution even under transient exposure time (10 ms) and low input power of incident laser (200 μW), which lead to obtain high-resolution single cell image within 30 s without inducing significant cell damage. The high resolution Raman image showed the distributions of gold nanoparticles for their targeted sites such as cytoplasm, mitochondria, or nucleus. The high speed Raman-based live cell imaging allowed us to monitor rapidly changing cell morphologies during cell death induced by the addition of highly toxic KCN solution to cells. These results strongly suggest that the use of SERS-active nanoparticle can greatly improve the current temporal resolution and image quality of Raman-based cell images enough to obtain the detailed cell dynamics and/or the responses of cells to potential drug molecules.
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DOI：
10.1073/pnas.0813327106
发表时间：
2009-08-11
影响因子：
11.1
作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
3.6
作者：
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通讯作者：
Hamaguchi, Hiro-o
影响因子：
10.8
作者：
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通讯作者：
Mahajan S