Age-Related Changes to Human Stratum Corneum Lipids Detected Using Time-of-Flight Secondary Ion Mass Spectrometry Following in Vivo Sampling.
Age-Related Changes to Human Stratum Corneum Lipids Detected Using Time-of-Flight Secondary Ion Mass Spectrometry Following in Vivo Sampling.
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体内采样后使用飞行时间二次离子质谱法检测人类角质层脂质与年龄相关的变化。
DOI：
10.1021/acs.analchem.5b04872
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发表时间：
2016
影响因子：
7.4
通讯作者：
Starr NJ
中科院分区：
文献类型：
--
作者：
Starr NJ
这项工作证明了从体内收集的样品中检测人类角质层 (SC) 脂质的数量和空间分布变化的能力。 SC 作为身体的主要屏障，防止外源物质的渗透。 SC 脂质成分的变化与屏障损伤和随之而来的皮肤病有关，因此监测和量化这种结构的变化非常重要。这项工作展示了首次报道的使用飞行时间二次离子质谱 (ToF-SIMS) 来评估人体 SC 随深度变化的生理变化。该技术提供了卓越的灵敏度和化学特异性，可以对从志愿者身上采集的单条胶带剥离样本进行分析。使用这种方法，我们能够成功识别因内在和外在（光）衰老而导致的人类 SC 的化学差异。从两个年龄组的女性（27 岁以下和绝经后）和紫外线照射不同的两个身体部位（前臂内侧和手背）收集样本，并使用多变量数据分析来确定差异。关键发现是甾醇胆固醇硫酸盐（一种膜稳定脂质）的空间分布与年龄相关的显着增加和变化。还观察到二十四烷酸 (C24:0) 和二十二烷酸 (C26:0) 的流行率发生显着变化。这项工作描述了之前未报道的人类 SC 中与年龄相关的化学变化，提供了对衰老机制的深入了解，这可能会改善药物和化妆品外用产品的设计。
This work demonstrates the ability to detect changes in both quantity and spatial distribution of human stratum corneum (SC) lipids from samples collected in vivo. The SC functions as the predominant barrier to the body, protecting against the penetration of xenobiotic substances. Changes to the SC lipid composition have been associated with barrier impairment and consequent skin disorders, and it is therefore important to monitor and quantify changes to this structure. This work demonstrates the first reported use of time-of-flight secondary ion mass spectrometry (ToF-SIMS) to assess physiological changes to human SC as a function of depth. This technique provides exceptional sensitivity and chemical specificity, allowing analysis of single tape stripped samples taken from volunteers. Using this methodology we were able to successfully identify chemical differences in human SC resulting from both intrinsic and extrinsic (photo) aging. Samples were collected from women of two age groups (under 27 and postmenopausal) and from two body sites with varying UV exposure (inner forearm and dorsal hand), and differences were identified using multivariate data analysis. The key finding was the significant aged-related increase and change in spatial distribution of the sterol cholesterol sulfate, a membrane stabilizing lipid. Significant changes in the prevalence of both lignoceric acid (C24:0) and hexacosanoic acid (C26:0) were also observed. This work describes previously unreported age-related chemical changes to human SC, providing an insight into aging mechanisms which may improve the design of both pharmaceutical and cosmetic topical products.
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影响因子：
3
作者：
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DOI：
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