Light scattering intensity as signal transducer to enhance the performance of immunoassay for Cronobacter detection in powdered infant formula
Light scattering intensity as signal transducer to enhance the performance of immunoassay for Cronobacter detection in powdered infant formula
复制标题
光散射强度作为信号传感器，可增强婴儿配方奶粉中克罗诺杆菌检测的免疫分析性能
DOI：
10.1016/j.snb.2021.130312
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发表时间：
2021-10
期刊：
影响因子：
--
通讯作者：
Xiong Yonghua
中科院分区：
文献类型：
--
作者：
Gao Bao;Ma Tongtong;Feng Lin;Huang Xiaolin;Chen Xuelan;Xiong Yonghua
本文提出利用银和金合金的光散射强度作为信号传感器，提高免疫分析法定量检测穆氏梭菌的灵敏度和动态线性。muytjensii）。通过在多枝晶胶体金表面生长银，制备了银金合金，即银包覆金纳米复合材料（AuNF@Ag）。银的生长是在银和葡萄糖存在下由尿素酶水解为尿素而引发的。由于结合了AuNF@Ag的光散射和酶催化信号放大的优点，所开发的光散射免疫分析方法具有较高的灵敏度。该方法的检出限为51 CFU/mL，比常规酶联免疫吸附试验（ELISA）的检出限（6.39 × 104 CFU/mL）低3个数量级。此外，该方法在3.4 × 102 ~ 3.4 × 109 CFU/mL范围内具有较宽的动态曲线，可用于C.与常规ELISA相比，该方法具有显著的上级优势（3.4 × 105 CFU/mL ~ 3.4 × 109 CFU/mL）。通过C. Muytjensii强化的PIF样品。批内、批间平均回收率在87.90%~ 107.91%之间，变异系数在2.72%~ 9.32%之间。这一发现表明，定量检测的准确性可接受的C。真实的PIF样品中的muytjensiiin。
Herein, we propose the light scattering intensity of silver and gold alloy as signal transducer to enhance the sensitivity and dynamic linearity of immunoassay for quantitative detection ofCronobacter muytjensii(C. muytjensii) in powered infant formula (PIF). Silver and gold alloy, namely, silver-coated gold nanocomposite (AuNF@Ag), was obtained by growing silver on the surface of multi-dendritic colloidal gold. The silver growth was triggered by the hydrolysis of urease to urea in the presence of silver nitrate and glucose. Owing to integrating the advantages of light scattering of AuNF@Ag and enzyme-catalyzed mediated signal amplification, the developed light scattering based immunoassay shows high sensitivity for detection ofC. muytjensii, with a detection limit of 51 CFU/mL, which is three orders of magnitude lower than that of conventional enzyme-linked immunosorbent assay (ELISA, 6.39 × 104CFU/mL). In addition, the proposed method also exhibits wide dynamic curve from 3.4 × 102CFU/mL to 3.4 × 109CFU/mL for quantitative detection ofC. muytjensii,and is significantly superior conventional ELISA (3.4 × 105CFU/mL to 3.4 × 109CFU/mL). The accuracy and precision of the proposed immunoassy were evaluated by analysis ofC. muytjensii-fortified PIF samples. The average recoveries of the intra- and inter-assays are within 87.90 %–107.91 %, and the coefficient of variation ranges from 2.72 %–9.32 %. This finding indicates an acceptable accuracy for quantitative detection ofC. muytjensiiin real PIF samples.
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