Protocol for using serial two-photon tomography to map cell types and cerebrovasculature at single-cell resolution in the whole adult mouse brain.
Protocol for using serial two-photon tomography to map cell types and cerebrovasculature at single-cell resolution in the whole adult mouse brain.
复制标题
DOI：
10.1016/j.xpro.2023.102048
复制
发表时间：
2023-03-17
期刊：
影响因子：
--
通讯作者：
Kim, Yongsoo
中科院分区：
文献类型：
--
作者：
Liwang, Josephine K.;Bennett, Hannah C.;Pi, Hyun-Jae;Kim, Yongsoo
在这里，我们提出了一个协议，使用串行双光子断层扫描（STPT）定量映射遗传定义的细胞类型和血管系统在整个成年小鼠大脑的单细胞分辨率。我们描述了脑组织的制备和样品包埋的细胞类型和血管STPT成像和图像处理使用MATLAB代码。我们详细介绍了细胞信号检测，血管跟踪和三维图像配准到解剖图谱的计算分析，这可以实现不同细胞类型的全脑映射。有关本方案使用和执行的完整详细信息，请参见Wu等人。（2022），Son et al.（2022），Newmaster et al.（2020），Kim等人（2017），和Ragan et al.（2012年）。使用连续双光子断层扫描（STPT）进行全小鼠脑成像的方案STPT成像的脑样本收集和包埋步骤定量细胞类型和脑血管映射的计算分析管道可以应用于不同细胞类型的全脑映射出版商说明：进行任何实验方案都需要遵守当地机构的实验室安全和伦理指南。在这里，我们提出了一个协议，使用串行双光子断层扫描（STPT）定量映射遗传定义的细胞类型和血管系统在整个成年小鼠大脑的单细胞分辨率。我们描述了脑组织的制备和样品包埋的细胞类型和血管STPT成像和图像处理使用MATLAB代码。我们详细介绍了细胞信号检测，血管跟踪和三维图像配准到解剖图谱的计算分析，这可以实现不同细胞类型的全脑映射。
Here, we present a protocol using serial two-photon tomography (STPT) to quantitatively map genetically defined cell types and cerebrovasculature at single-cell resolution across the entire adult mouse brain. We describe the preparation of brain tissue and sample embedding for cell type and vascular STPT imaging and image processing using MATLAB codes. We detail the computational analyses for cell signal detection, vascular tracing, and three-dimensional image registration to anatomical atlases, which can be implemented for brain-wide mapping of different cell types. For complete details on the use and execution of this protocol, please refer to Wu et al. (2022), Son et al. (2022), Newmaster et al. (2020), Kim et al. (2017), and Ragan et al. (2012). A protocol to use serial two-photon tomography (STPT) for whole mouse brain imaging Steps for brain sample collection and embedding for STPT imaging Computational analysis pipelines for quantitative cell type and cerebrovascular mapping Can be applied for brain-wide mapping of different cell types Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. Here, we present a protocol using serial two-photon tomography (STPT) to quantitatively map genetically defined cell types and cerebrovasculature at single-cell resolution across the entire adult mouse brain. We describe the preparation of brain tissue and sample embedding for cell type and vascular STPT imaging and image processing using MATLAB codes. We detail the computational analyses for cell signal detection, vascular tracing, and three-dimensional image registration to anatomical atlases, which can be implemented for brain-wide mapping of different cell types.
登录
查看更多内容
影响因子：
1.5
作者：
Cuttler, Anne S.;LeClair, Renee J.;Stohn, J. Patrizia;Wang, Qiaozeng;Sorenson, Christine M.;Liaw, Lucy;Lindner, Volkhard
通讯作者：
Lindner, Volkhard
影响因子：
8.8
作者：
Wu, Yuan-ting;Bennett, Hannah C.;Chon, Uree;Vanselow, Daniel J.;Zhang, Qingguang;Munoz-Castarieda, Rodrigo;Cheng, Keith C.;Osten, Pavel;Drew, Patrick J.;Kim, Yongsoo
通讯作者：
Kim, Yongsoo
DOI：
10.1523/jneurosci.3287-09.2009
发表时间：
2009-11-18
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Tsai PS;Kaufhold JP;Blinder P;Friedman B;Drew PJ;Karten HJ;Lyden PD;Kleinfeld D
通讯作者：
Kleinfeld D
影响因子：
64.5
作者：
Kim Y;Yang GR;Pradhan K;Venkataraju KU;Bota M;García Del Molino LC;Fitzgerald G;Ram K;He M;Levine JM;Mitra P;Huang ZJ;Wang XJ;Osten P
通讯作者：
Osten P
影响因子：
48
作者：
Ragan, Timothy;Kadiri, Lolahon R.;Venkataraju, Kannan Umadevi;Bahlmann, Karsten;Sutin, Jason;Taranda, Julian;Arganda-Carreras, Ignacio;Kim, Yongsoo;Seung, H. Sebastian;Osten, Pavel
通讯作者：
Osten, Pavel