Involvement of Thyroid Hormones in the Expression of MHC class I Antigens During Ontogeny in Xenopus
Involvement of Thyroid Hormones in the Expression of MHC class I Antigens During Ontogeny in Xenopus
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甲状腺激素参与爪蟾个体发育过程中 MHC I 类抗原表达
DOI：
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
W. Green
中科院分区：
文献类型：
--
作者：
L. Rollins‐Smith;M. Flajnik;P. Blair;A. Davis;W. Green
主要组织相容性复合体(MHC)是编码脊椎动物免疫系统所有主要功能中心的产物的基因簇。在所有被检查的脊椎动物群体中都可以找到MHC的证据，除了无颌鱼。在个体发育早期，MHC I类和II类抗原的表达对于能够区分自我和非我的T淋巴细胞的发育至关重要。由于MHC在T细胞发育中的重要作用，我们对非洲爪蛙MHC表达的个体发育进行了研究。以往对非洲爪哇MHC-I类抗原表达的研究表明，在变态高潮之前，蝌蚪组织中几乎不存在MHC-I类抗原。因此，我们研究了甲状腺激素(TH)在诱导第I类基因表达中的可能作用。用流式细胞术检测到，在变态前期55-58期，未处理的蛙的脾细胞和红细胞上可检测到少量第I类基因的表达，并在变性高潮结束时显著增加。因此，变态与I类基因表达强度的增加有关。无论是对变态的抑制还是对变态的加速，都不会改变I类基因表达的开始时间。然而，对变态的抑制阻止了成体细胞群体中I类表达的增加。由于TH处理的青蛙没有加速表达，或者在变态抑制的青蛙中没有延迟表达，这不太可能是诱导表达的直接发育信号，尽管它们似乎是变态时上调I类表达所必需的。在不同的细胞亚群中表达模式的差异表明在个体发育过程中I类抗原表达的调节是复杂的模式。
The major histocompatibility complex (MHC) is a cluster of genes encoding products central to all major functions of the vertebrate immune system. Evidence for an MHC can be found in all vertebrate groups that have been examined except the jawless fishes. Expression of MHC class I and class II antigens early in ontogeny is critically important for development of T lymphocytes capable of discriminating self from nonself. Because of this essential role in T-cell development, the ontogeny of MHC expression in the South African clawed frog, Xenopus laevis, was studied. Previous studies of MHC class I expression in Xenopus laevis suggested that class I antigens are virtually absent from tadpole tissues until climax of metamorphosis. We therefore examined the possible role of thyroid hormones (TH) in the induction of class I. By flow cytometry, a small amount of class I expression was detectable on splenocytes and erythrocytes in untreated frogs at prometamorphic stages 55-58, and the amount increased significantly at the conclusion of metamorphic climax. Thus, metamorphosis is associated with increased intensity of class I expression. Neither inhibition nor acceleration of metamorphosis altered the timing of onset of class I expression. However, inhibition of metamorphosis prevented the increase in class I expression characteristic of adult cell populations. Because expression was not accelerated in TH-treated frogs or delayed in metamorphosis-inhibited frogs, it is unlikely that TH are the direct developmental cues that induce expression, although they seem to be required for the upregulation of class I expression occurring at metamorphosis. Differences in the pattern of expression in different subpopulations of cells suggest a complex pattern of regulation of expression of class I antigens during ontogeny.
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MHC 的进化：爪蟾（青蛙）II 类分子的抗原性和异常组织分布。
影响因子：
3.6
作者：
Flajnik,MF;Ferrone,S;Cohen,N;DuPasquier,L
通讯作者：
DuPasquier,L
在青蛙个体发育过程中，脾细胞对 PHA 和 Con A 的反应性先于胸腺细胞。
影响因子：
6.4
作者：
Rollins-Smith,LA;Parsons,SC;Cohen,N
通讯作者：
Cohen,N
DOI：
10.1073/pnas.88.2.537
发表时间：
1991-01-01
影响因子：
11.1
作者：
FLAJNIK, MF;CANEL, C;KASAHARA, M
通讯作者：
KASAHARA, M
马 T 淋巴细胞表达 MHC II 类抗原。
DOI：
10.1111/j.1744-313x.1986.tb01119.x
发表时间：
1986
期刊：
Journal of immunogenetics
影响因子：
--
作者：
Crepaldi,T;Crump,A;Newman,M;Ferrone,S;Antczak,DF
通讯作者：
Antczak,DF
甲状腺素驱动的性早熟对早期甲状腺切除青蛙成年型同种异体移植排斥反应成熟的影响。
DOI：
10.1111/j.1432-0436.1988.tb00719.x
发表时间：
1988
期刊：
Differentiation; research in biological diversity
影响因子：
--
作者：
Rollins-Smith,LA;Parsons,SC;Cohen,N
通讯作者：
Cohen,N