Coinfection of Clonorchis sinensis and hepatitis B virus: clinical liver indices and interaction in hepatic cell models.
Coinfection of Clonorchis sinensis and hepatitis B virus: clinical liver indices and interaction in hepatic cell models.
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华支睾吸虫与乙型肝炎病毒共感染：临床肝脏指标及肝细胞模型中的相互作用
DOI：
10.1186/s13071-022-05548-5
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发表时间：
2022-12-12
影响因子：
3.2
通讯作者：
中科院分区：
文献类型：
--
作者：
在中国，乙型肝炎病毒（HBV）感染者常见于华支睾吸虫（一种吸虫）高发地区。已发表的研究报告称，乙肝的进展受同时感染中华支原体的影响。分析了72例中华梭菌合并HBV患者（单独感染或合并感染）和29例健康个体的临床资料。我们还用中华绒螯蟹成虫（CsTPs）和hbv阳性血清的总蛋白培养肝星状细胞株LX-2。此外，采用抗病毒药物恩替卡韦（ETV）、cstp和anti-C治疗人肝母细胞瘤细胞株HepG2.2.15。中药吡喹酮（PZQ）。我们的临床数据表明，合并感染患者的谷丙转氨酶（ALT）、天冬氨酸转氨酶（AST）、总胆红素（TB）和透明质酸（HA）水平明显高于单纯感染HBV的患者。在细胞模型中，与HBV阳性血清（HBV组）孵育LX-2细胞模型相比，CsTP和HBV阳性血清（CsTP+HBV组）孵育LX-2细胞模型中α -平滑肌肌动蛋白和I型、III型胶原转录物显著升高，而CsTP+HBV组肿瘤坏死因子-α、白细胞介素(IL)-1β和IL-6 mrna水平明显降低。在HepG2.2.15细胞中，ETV和CsTPs处理的HBV表面抗原和乙型肝炎e抗原水平高于ETV组和ETV、CsTPs和PZQ混合处理的细胞。这些结果证实，中华梭菌与HBV共同感染可加重肝纤维化的进展。cstp可能促进HBV感染个体的肝脏慢性炎症，导致肝纤维化的发展。在线版本包含补充材料，可在10.1186/s13071-022-05548-5获得。
In China, people infected with hepatitis B virus (HBV) are commonly found in areas with a high prevalence of Clonorchis sinensis, a trematode worm. Published studies have reported that the progression of hepatitis B is affected by coinfection C. sinensis. Clinical data from a total of 72 patients with C. sinensis and HBV (as sole infection or with coinfections) and 29 healthy individuals were analysed. We also incubated the hepatic stellate cell line LX-2 with total proteins from C. sinensis adult worms (CsTPs) and HBV-positive sera. In addition, the human hepatoblastoma cell line HepG2.2.15 was treated with the antiviral drug entecavir (ETV), CsTPs and the anti-C. sinensis drug praziquantel (PZQ). Our clinical data indicated that the levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin (TB) and hyaluronic acid (HA) were significantly higher in patients with coinfection than in those infected with HBV only. In cell models, compared with the model in which LX-2 cells were incubated with HBV-positive sera (HBV group), transcripts of alpha-smooth muscle actin and types I and III collagen were significantly elevated in the models of LX-2 cells treated with CsTPs and HBV-positive sera (CsTP+HBV group), while the messenger RNA levels of tumour necrosis factor-α, interleukin (IL)-1β and IL-6 in the CsTP+HBV group were clearly lower. The HBV surface antigen and hepatitis B e-antigen levels were higher in the HepG2.2.15 cells treated with ETV and CsTPs than in those in the ETV group and in the cells administered a mixture of ETV, CsTPs and PZQ. These results confirmed that C. sinensis and HBV coinfection could aggravate the progression of liver fibrosis. CsTPs might promote chronic inflammation of the liver in individuals with HBV infection, resulting in the development of hepatic fibrosis. The online version contains supplementary material available at 10.1186/s13071-022-05548-5.
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影响因子：
2.7
作者：
Deng, Zhuo-Hui;Fang, Yue-Yi;Liu, Meng-Ran
通讯作者：
Liu, Meng-Ran
靶向激活的肝星状细胞 (aHSC) 进行肝纤维化成像
影响因子：
3.2
作者：
Li D;He L;Guo H;Chen H;Shan H
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肝病中的透明质酸浓度。
影响因子：
4.6
作者：
Gudowska, Monika;Gruszewska, Ewa;Panasiuk, Anatol;Cylwik, Bogdan;Flisiak, Robert;Aewiderska, Magdalena;Szmitkowski, Maciej;Chrostek, Lech
通讯作者：
Chrostek, Lech
影响因子：
4.2
作者：
Chai JY
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Chai JY
影响因子：
3
作者：
Jiang, Dapeng;Jiang, Zhitao;Li, Zhaozhu
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Li, Zhaozhu