Comparison of the vaginal microbiota diversity of women with and without human papillomavirus infection: a cross-sectional study.
Comparison of the vaginal microbiota diversity of women with and without human papillomavirus infection: a cross-sectional study.
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DOI：
10.1186/1471-2334-13-271
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发表时间：
2013-06-10
影响因子：
3.7
通讯作者：
Chen X
中科院分区：
文献类型：
--
作者：
Gao W;Weng J;Gao Y;Chen X
女性生殖道是人体重要的细菌栖息地，阴道微生物群对阴道健康起着至关重要的作用。阴道微生物群的改变每年影响数百万女性，并与许多不良健康后果有关，包括人乳头瘤病毒（HPV）感染。然而，以前的研究主要集中在细菌性阴道病和HPV感染之间的关联。关于HPV感染中阴道微生物群落的组成知之甚少。本研究旨在调查HPV感染是否与阴道微生物群的多样性和组成相关。共有70名宫颈细胞学检查正常的健康女性（32名HPV阴性和38名HPV阳性）入选本研究。非培养依赖性聚合酶链反应-变性梯度凝胶电泳用于测量所有受试者阴道微生物群的多样性和组成。我们发现HPV阳性女性阴道微生物群的生物多样性显着增加（p < 0.001）。乳杆菌属（Lactobacillus）包括L. gallinarum，鸡乳属L. iners和L. gasseri是主要的属，在所有妇女中均检测到。HPV阳性和阴性妇女的L。gallinarum（p = 0.775）或L. iners（p = 0.717），而L.在HPV阳性妇女中发现gasseri的频率显著较高（p = 0.005）。在HPV阳性女性中也发现了更高频率的迷走加德纳菌（p = 0.031）。树状图显示，来自两组的阴道微生物群具有不同的特征。我们的研究是第一次系统评估阴道微生物群与HPV感染之间的关联，我们已经证明，与HPV阴性女性相比，HPV阳性女性的细菌多样性更复杂，阴道微生物群的组成也不同。
The female genital tract is an important bacterial habitat of the human body, and vaginal microbiota plays a crucial role in vaginal health. The alteration of vaginal microbiota affects millions of women annually, and is associated with numerous adverse health outcomes, including human papillomavirus (HPV) infection. However, previous studies have primarily focused on the association between bacterial vaginosis and HPV infection. Little is known about the composition of vaginal microbial communities involved in HPV acquisition. The present study was performed to investigate whether HPV infection was associated with the diversity and composition of vaginal microbiota. A total of 70 healthy women (32 HPV-negative and 38 HPV-positive) with normal cervical cytology were enrolled in this study. Culture-independent polymerase chain reaction-denaturing gradient gel electrophoresis was used to measure the diversity and composition of vaginal microbiota of all subjects. We found significantly greater biological diversity in the vaginal microbiota of HPV-positive women (p < 0.001). Lactobacillus, including L. gallinarum, L. iners and L. gasseri, was the predominant genus and was detected in all women. No significant difference between HPV-positive and HPV-negative women was found for the frequency of detection of L. gallinarum (p = 0.775) or L. iners (p = 0.717), but L. gasseri was found at a significantly higher frequency in HPV-positive women (p = 0.005). Gardnerella vaginalis was also found at a significantly higher frequency in HPV-positive women (p = 0.031). Dendrograms revealed that vaginal microbiota from the two groups had different profiles. Our study is the first systematic evaluation of an association between vaginal microbiota and HPV infection, and we have demonstrated that compared with HPV-negative women, the bacterial diversity of HPV-positive women is more complex and the composition of vaginal microbiota is different.
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影响因子：
9.4
作者：
Kim, Tae Kyung;Thomas, Susan M.;Wilson, Brenda A.
通讯作者：
Wilson, Brenda A.
影响因子：
3.6
作者：
Ling, Zongxin;Liu, Xia;Xiang, Charlie
通讯作者：
Xiang, Charlie
影响因子：
7.6
作者：
Li, Y;Ku, CYS;Caufield, PW
通讯作者：
Caufield, PW
影响因子：
3.7
作者：
Gillet E;Meys JF;Verstraelen H;Bosire C;De Sutter P;Temmerman M;Broeck DV
通讯作者：
Broeck DV
影响因子：
3.1
作者：
Cherpes, Thomas L.;Hillier, Sharon L.;Krohn, Marijane A.
通讯作者：
Krohn, Marijane A.