Suppressive role of B cells in chronic colitis of T cell receptor alpha mutant mice.
Suppressive role of B cells in chronic colitis of T cell receptor alpha mutant mice.
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DOI：
10.1084/jem.186.10.1749
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发表时间：
1997-11-17
期刊：
影响因子：
--
通讯作者：
Bhan AK
中科院分区：
文献类型：
--
作者：
Mizoguchi A;Mizoguchi E;Smith RN;Preffer FI;Bhan AK
通过建立缺乏B细胞的双突变小鼠（TCR-α - / - ×免疫球蛋白(Ig)μ - / -），探讨抗体（Abs）在T细胞受体(TCR)-α - / -小鼠慢性结肠炎发生中的作用。TCR-α−/−× Igμ−/−小鼠自发性结肠炎发生年龄更早，结肠炎较TCR-α−/−小鼠严重。用TCR-α−/−× Igμ−/−小鼠的肠系膜淋巴结（MLN）细胞转染重组激活基因-1 （RAG-1−/−）小鼠，诱导结肠炎。将TCR-α−/−小鼠纯化的B细胞与MLN细胞混合后，rag1 -1−/−小鼠未发生结肠炎。从TCR-α - / -小鼠中纯化的Ig和与结肠上皮细胞反应的单克隆自身抗体混合使用，可导致TCR-α - / - × Igμ - / -小鼠结肠炎的减弱。与Igμ−/−小鼠和TCR-α−/−小鼠相比，TCR-α−/−小鼠结肠、MLN和脾脏的凋亡细胞增多。TCR-α−/−小鼠Ig纯化后，TCR-α−/−× Igμ−/−小鼠的凋亡细胞数量明显减少。这些发现表明，虽然结肠炎的发生不需要B细胞，但B细胞和igg（自身抗体）可能通过影响凋亡细胞的清除来抑制结肠炎。
The role of antibodies (Abs) in the development of chronic colitis in T cell receptor (TCR)-α−/− mice was explored by creating double mutant mice (TCR-α−/− × immunoglobulin (Ig)μ−/−), which lack B cells. TCR-α−/− × Igμ−/− mice spontaneously developed colitis at an earlier age, and the colitis was more severe than in TCR-α−/− mice. Colitis was induced in recombination-activating gene-1 (RAG-1−/−) mice by the transfer of mesenteric lymph node (MLN) cells from TCR-α−/− × Igμ−/− mice. When purified B cells from TCR-α−/− mice were mixed with MLN cells before cell transfer, colitis did not develop in RAG-1−/− mice. Administration of the purified Ig from TCR-α−/− mice and a mixture of monoclonal autoAbs reactive with colonic epithelial cells led to attenuation of colitis in TCR-α−/− × Igμ−/− mice. Apoptotic cells were increased in the colon, MLN, and spleen of TCR-α−/− × Igμ−/− mice as compared to Igμ−/− mice and TCR-α−/− mice. Administration of the purified Ig from TCR-α−/− mice into TCR-α−/− × Igμ−/− mice led to decrease in the number of apoptotic cells. These findings suggest that although B cells are not required for the initiation of colitis, B cells and Igs (autoAbs) can suppress colitis, presumably by affecting the clearance of apoptotic cells.
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影响因子：
15.3
作者：
Gilligan, H M;Bredy, B;Brady, H R;Hebert, M J;Slayter, H S;Xu, Y;Rauch, J;Shia, M A;Koh, J S;Levine, J S
通讯作者：
Levine, J S
DOI：
10.1084/jem.182.5.1567
发表时间：
1995-11-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Ma A;Datta M;Margosian E;Chen J;Horak I
通讯作者：
Horak I
影响因子：
5.4
作者：
BRUNO, L;ROCHA, B;RODEWALD, HR
通讯作者：
RODEWALD, HR
影响因子：
15.3
作者：
Mizoguchi, A;Mizoguchi, E;Bhan, AK
通讯作者：
Bhan, AK
影响因子：
64.8
作者：
MOMBAERTS, P;CLARKE, AR;TONEGAWA, S
通讯作者：
TONEGAWA, S