Triggered micropore-forming bioprinting of porous viscoelastic hydrogels.
Triggered micropore-forming bioprinting of porous viscoelastic hydrogels.
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DOI：
10.1039/d0mh00813c
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发表时间：
2020-09-01
影响因子：
13.3
通讯作者：
Mongeau L
中科院分区：
文献类型：
--
作者：
Bao G;Jiang T;Ravanbakhsh H;Reyes A;Ma Z;Strong M;Wang H;Kinsella JM;Li J;Mongeau L
模拟宿主组织的结构和力学的细胞负载支架对于广泛的生物医学应用是重要的，但对生物打印仍然具有挑战性。为了解决这些挑战，我们报告了一种名为触发微孔形成生物打印的新方法。该方法可以产生特定结构的细胞负载支架和不同大小的相互连接的孔，包括许多细胞类型。生物打印支架的粘弹性可以与生物组织的粘弹性相匹配，并且可以独立于孔隙率和硬度进行调节。生物打印支架还表现出卓越的机械稳定性，尽管孔隙率很高。生物打印方法和由此得到的支架支持细胞的扩散、迁移和增殖。3D生物打印系统在声带组织工程和作为体外癌症模型方面的潜力被证明。其他可能的应用还包括组织修复、再生医学、芯片器官、药物筛选、器官移植和疾病建模。这项工作报告了一种新的三维生物打印层次化细胞支架的方法，具有前所未有的多孔性和粘弹性属性的组合。
Cell-laden scaffolds of architecture and mechanics that mimic those of the host tissues are important for a wide range of biomedical applications but remain challenging to bioprint. To address these challenges, we report a new method called triggered micropore-forming bioprinting. The approach can yield cell-laden scaffolds of defined architecture and interconnected pores over a range of sizes, encompassing that of many cell types. The viscoelasticity of the bioprinted scaffold can match that of biological tissues and be tuned independently of porosity and stiffness. The bioprinted scaffold also exhibits superior mechanical robustness despite high porosity. The bioprinting method and the resulting scaffolds support cell spreading, migration, and proliferation. The potential of the 3D bioprinting system is demonstrated for vocal fold tissue engineering and as an in vitro cancer model. Other possible applications are foreseen for tissue repair, regenerative medicine, organ-on-chip, drug screening, organ transplantation, and disease modeling. This work reports a novel approach to 3D-bioprint hierarchical cell-laden scaffolds with an unprecedented combination of porous and viscoelastic properties.
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影响因子：
13.6
作者：
Hinton TJ;Jallerat Q;Palchesko RN;Park JH;Grodzicki MS;Shue HJ;Ramadan MH;Hudson AR;Feinberg AW
通讯作者：
Feinberg AW
组织模拟的纳米纤维纤维杂种可注射的水凝胶，用于潜在的软组织工程应用。
影响因子：
4.6
作者：
Latifi N;Asgari M;Vali H;Mongeau L
通讯作者：
Mongeau L
影响因子：
9.7
作者：
Beduer, A.;Piacentini, N.;Braschler, T.
通讯作者：
Braschler, T.
影响因子：
41.2
作者：
Chaudhuri O;Gu L;Klumpers D;Darnell M;Bencherif SA;Weaver JC;Huebsch N;Lee HP;Lippens E;Duda GN;Mooney DJ
通讯作者：
Mooney DJ
影响因子：
46.9
作者：
Kang, Hyun-Wook;Lee, Sang Jin;Atala, Anthony
通讯作者：
Atala, Anthony