A tissue-mimetic nano-fibrillar hybrid injectable hydrogel for potential soft tissue engineering applications.
A tissue-mimetic nano-fibrillar hybrid injectable hydrogel for potential soft tissue engineering applications.
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组织模拟的纳米纤维纤维杂种可注射的水凝胶，用于潜在的软组织工程应用。
DOI：
10.1038/s41598-017-18523-3
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发表时间：
2018-01-18
影响因子：
4.6
通讯作者：
Mongeau L
中科院分区：
文献类型：
--
作者：
Latifi N;Asgari M;Vali H;Mongeau L
虽然I型胶原蛋白（Col-I）通常被用作生物材料的结构成分，但在许多软组织中普遍存在的另一种形成胶原蛋白的原纤维III型胶原蛋白（Col-III）以前尚未被使用。在本研究中，研究了一种可注射水凝胶的新概念，该水凝胶具有异型胶原原纤维的半互穿聚合物网络，具有组织特异性的coli - iii和coli比例，在乙二醇-壳聚糖基质中。Col-III作为新型水凝胶的一个组成部分被引入，其灵感来自于它在许多软组织中与col - 1共存，它在直径和力学方面对col - 1纤维形成的影响，以及它在调节疤痕形成中的既定作用。该水凝胶具有纳米纤维状多孔结构，在连续动态刺激下具有机械稳定性。研究发现，与同类透明质酸基水凝胶相比，它的半衰期约为35天，并且在活力、代谢活性、粘附和迁移方面支持细胞植入。研究了纯Col-III原纤维在乙二醇-壳聚糖基质中的具体情况。所提出的水凝胶满足软组织工程应用的许多基本要求，特别是对于机械缺陷组织，如声带和心脏瓣膜。
While collagen type I (Col-I) is commonly used as a structural component of biomaterials, collagen type III (Col-III), another fibril forming collagen ubiquitous in many soft tissues, has not previously been used. In the present study, the novel concept of an injectable hydrogel with semi-interpenetrating polymeric networks of heterotypic collagen fibrils, with tissue-specific Col-III to Col-I ratios, in a glycol-chitosan matrix was investigated. Col-III was introduced as a component of the novel hydrogel, inspired by its co-presence with Col-I in many soft tissues, its influence on the Col-I fibrillogenesis in terms of diameter and mechanics, and its established role in regulating scar formation. The hydrogel has a nano-fibrillar porous structure, and is mechanically stable under continuous dynamic stimulation. It was found to provide a longer half-life of about 35 days than similar hyaluronic acid-based hydrogels, and to support cell implantation in terms of viability, metabolic activity, adhesion and migration. The specific case of pure Col-III fibrils in a glycol-chitosan matrix was investigated. The proposed hydrogels meet many essential requirements for soft tissue engineering applications, particularly for mechanically challenged tissues such as vocal folds and heart valves.
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影响因子：
2.4
作者：
Chan, RW;Titze, IR
通讯作者：
Titze, IR
影响因子：
2.9
作者：
Cuttle, L;Nataatmadja, M;Hayes, MT
通讯作者：
Hayes, MT
影响因子：
2.1
作者：
BETZ, P;NERLICH, A;EISENMENGER, W
通讯作者：
EISENMENGER, W
影响因子：
6
作者：
Croisier, Florence;Jerome, Christine
通讯作者：
Jerome, Christine
影响因子：
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作者：
Attallah, Abdelfattah M.;Mosa, Tamer E.;Shaker, Yehia M.
通讯作者：
Shaker, Yehia M.