Research Note: Repetitive element-based polymerase chain reaction genotyping improves efficiency of Salmonella surveillance in a model broiler production system.
Research Note: Repetitive element-based polymerase chain reaction genotyping improves efficiency of Salmonella surveillance in a model broiler production system.
复制标题
研究报告：基于重复元件的聚合酶链反应基因分型提高了模型肉鸡生产系统中沙门氏菌监测的效率。
DOI：
10.1016/j.psj.2019.12.048
复制
发表时间：
2020-05
期刊：
影响因子：
4.4
通讯作者：
Brake J
中科院分区：
文献类型：
--
作者：
Walker GK;Suyemoto MM;Borst LB;Brake J
测定了从家禽及其环境分离的沙门氏菌的遗传亲缘关系和抗菌药物敏感性。在同一家禽研究农场饲养1只肉种鸡群（BBF1）和2只肉种鸡群（BF1和BF2），历时1.75年。从BBF1中获得孵化蛋以生产BF1雏鸡，而BF2雏鸡是单独的，未取样的肉鸡繁殖群的后代。BF1和BF2在相同的饲养设施中饲养，但间隔6个月。沙门氏菌分离物采集方法为：粪便取样（BF1）、盲肠切除（BF1和BF2）或肛肠拭子（BBF1）。血清分型鉴定肠沙门氏菌亚种。BBF1和肠链球菌亚群中的肠血清性碱性氨基酸（SA）。小肠Senftenberg （SS）在BF1和BF2。利用（GTG）5引物进行基因型指纹图谱分析，结果显示BF1和BF2分离株具有同源性。对于每个分离株，使用敏化试验板确定了27种抗菌剂的最低抑制浓度，该板的处方针对家禽生产中使用的抗菌剂或用于控制革兰氏阴性病原体的抗菌剂。3株鸡对克林霉素、红霉素、新生物霉素、青霉素和酒石酸泰乐霉素耐药，对阿奇霉素、氟苯尼考和大霉素耐药。这些数据表明，Altona血清型和Senftenberg血清型由家禽携带，后者似乎在肉鸡群中持续存在，并且在综合研究操作中，两种血清型具有相似的抗菌药物敏感性模式。在多种沙门氏菌分离株的情况下，将基因型指纹图谱方法与代表性分离株的血清分型相结合将减少血清分型所需的样品数量，并更清楚地确定分离株的亲缘关系。这些方法有助于在家禽生产系统中进行有效监测，从而允许实施精确的沙门氏菌控制措施。
The genetic relatedness and antimicrobial susceptibility profiles of Salmonella isolated from poultry and their environment were determined. One broiler breeder flock (BBF1) and 2 broiler flocks (BF1 and BF2) were reared over a 1.75-year period on the same poultry research farm. Hatching eggs were obtained from BBF1 to produce BF1 chicks, while BF2 chicks were progeny of a separate, unsampled broiler breeder flock. BF1 and BF2 were reared in the same housing facilities but 6 mo apart. Salmonella isolates were collected via litter sock sampling (BF1), cecal excision (BF1 and BF2), or cloacal swabs (BBF1). Serotyping identified Salmonella enterica subsp. enterica serovar Altona (SA) in BBF1 and S. enterica subsp. enterica serovar Senftenberg (SS) in BF1 and BF2. Genotypic fingerprinting was achieved with Rep-PCR using the (GTG)5 primer and revealed sequence homology among Senftenberg isolates from BF1 and BF2. For each isolate, the minimum inhibitory concentration was determined for 27 antimicrobial agents using Sensititre plates with formularies specific to antimicrobials used in poultry production or those used to control gram negative pathogens. Isolates from the 3 flocks were resistant to clindamycin, erythromycin, novobiocin, penicillin, and tylosin tartrate and demonstrated intermediate resistance to azithromycin, florfenicol, and spectinomycin. These data demonstrated that serovar Altona and Senftenberg were harbored by poultry, the latter appeared to persist in broiler flocks, and both serotypes shared similar patterns of antimicrobial susceptibility in an integrated research operation. In the case of multiple Salmonella isolates, combining genotypic fingerprinting methods with serotyping of representative isolates would reduce the number of samples required for serotyping and more clearly identify relatedness of isolates. These methods facilitate effective surveillance in poultry production systems, thus allowing for implementation of precise Salmonella control measures.
登录
查看更多内容
影响因子：
2.6
作者：
Marin, C.;Balasch, S.;Lainez, M.
通讯作者：
Lainez, M.
影响因子：
4.4
作者：
Caraway, C. T.;Walker, G. K.;Brake, J.
通讯作者：
Brake, J.
影响因子：
3.2
作者：
Liljebjelke KA;Hofacre CL;White DG;Ayers S;Lee MD;Maurer JJ
通讯作者：
Maurer JJ
影响因子：
2.8
作者：
Liljebjelke, Karen A.;Hofacre, Charles L.;Maurer, John J.
通讯作者：
Maurer, John J.
影响因子：
4.4
作者：
Oh, J-Y.;Kang, M-S.;Kwon, Y-K.
通讯作者：
Kwon, Y-K.